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豚鼠气管平滑肌细胞培养
引用本文:郝智慧,赵厚德,张予阳,王笑笑.豚鼠气管平滑肌细胞培养[J].实验动物科学,2011,28(5):15-18.
作者姓名:郝智慧  赵厚德  张予阳  王笑笑
作者单位:1. 沈阳药科大学生命科学与生物制药学院实验动物中心,沈阳,110016
2. 沈阳药科大学生命科学与生物制药学院药理系,沈阳,110016
摘    要:目的建立一种简便、快捷的豚鼠气管平滑肌细胞培养方法。方法幼年豚鼠气管平滑肌组织块原代培养法。结果豚鼠气管平滑肌组织块贴壁5~8 d后可见细胞自组织块周围长出,细胞长梭形,核位于中央,呈辐射状向四周生长。两周后细胞长满瓶壁。经α-平滑肌肌动蛋白单克隆抗体(α-smooth muscle actin monoclonal antibody,α-SMA)免疫组化染色鉴定,获得的细胞为平滑肌细胞。结论豚鼠气管平滑肌组织块培养法可快速获得数量充足的气管平滑肌细胞。

关 键 词:气管平滑肌  细胞培养  豚鼠

Primary Culture of Tracheal Smooth Muscle Cells of Guinea Pigs
HAO Zhi-hui,ZHAO Hou-de,ZHANG Yu-yang,WANG Xiao-xiao.Primary Culture of Tracheal Smooth Muscle Cells of Guinea Pigs[J].Shiyan Dongwu Kexue,2011,28(5):15-18.
Authors:HAO Zhi-hui  ZHAO Hou-de  ZHANG Yu-yang  WANG Xiao-xiao
Institution:HAO Zhi-hui1,ZHAO Hou-de1,ZHANG Yu-yang2,WANG Xiao-xiao2(1.Laboratory Animal Center,Shenyang Pharmaceutical University,Shenyang 110016,China)(2.Department of pharmacology,China)
Abstract:Objective To establish a method of primary culture tracheal smooth muscle(TSM) cells of guinea pigs.Methods Cultured TSM cells of guinea pigs by tissue cultivation and identified TSM cells by the method of immunocytochemistry stain with specific α-smooth muscle actin antibody.Results Cells in fusiform or polygon shape erupted around the pieces of smooth muscle tissue and grew and proliferated radiat with a nucleus lying in the middle of cytoplasm in 5~8 days.The immunocytochemical stain of the monoclonal antibody of anti-smooth muscle α-actin was positive and the purity of the cells was above 95%,which indicated that most of cultured cells were smooth muscle cells.Conclusions The method of culture TSM cells of guinea pig is successful and can be applied.
Keywords:tracheal smooth muscle(TSM)  cells culture  guinea pigs  
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