裸小鼠的繁殖及在肿瘤学中的应用

目的　总结了八年实验动物裸小鼠繁殖和应用。方法　用随机抽取 2 0窝BALB/c裸小鼠繁殖生育情况分析和 5只BALB/c母鼠 (nu/ +)四胎生育情况比较。结果　BALB/c裸小鼠平均每胎产仔 9 35只 ,用nu/ +雌与nu/nu雄交配方法繁殖 ,平均每胎所得纯合子仔鼠能占仔数的 5 5 0 % ,离乳率 97% ,1～ 4胎生育恒定 ,一年存活率达10 0 % ,最长生存期可达 75 2d。微生物检测结果未发现致病菌 ,寄生虫等。结论　空气过滤十万级环境加上生物层流柜达一万级的条件 ,只要严格执行无菌操作 ,裸小鼠繁殖可基本达到SPF要求。

Reproduction of Nude Mice and Their Application in Experimental Oncology

Objective To summarize the experience of the reproduction and application of the experimental animal nude mice for 8 years in our laboratory. Methods Randomly drawing out 20 litters of BALB/c nude mice and 5 maternal nude mice.analyze t heir generational state. Results Averagely, one BALB/c nude mou se gives birth to 9.35 mice every time. Female nu/+ and male nu/nu crossbreeding, 55.0 per cent of offspring are nu/ nu homozygous mice,and 97.0 per cent of offspring survives after ablactation.From the first-born to forth-born,the fecundity is steady, the survival rate for 1 year is 100 per cent,and the longe st survival period is 752 days.Microbiological detection couldn’t find any pathogen or parasite whic h should be controlled. Conclusion Under the condition of 100?000 grade air filtrating and in 10?000 grade lamina r flowing cabinet,with strict aseptic manipulation,we bred qualified specific pathogen free(SPF) nude mice.