顺铂致贵州小型猪呕吐模型

目的建立贵州小型猪恶心呕吐的动物模型。方法实验动物随机分为3组,用不同剂量顺铂一次性腹腔注射,观察8h内出现恶心和呕吐情况。结果在观察期,对照组动物未出现恶心呕吐表现,小剂量组(3 mg/kg)仅有1/4的动物在给药后382min出现恶心而未有呕吐,大剂量组(4 mg/kg)全部动物均出现恶心和呕吐,恶心出现的平均时间为68.25 min,出现呕吐的平均时间为138 min。结论贵州小型猪对顺铂引起的恶心和呕吐反应呈显著的剂量反应关系,反应均一,提示贵州小型猪可作为一种新的疾病模型实验动物,用于呕吐机制、药物和针灸抗呕吐的研究。

Vomiting Model Induced by Cisplatin in Guizhou Miniature Pig

Objective To establish me model of nausea and vomiting in Guizhou miniature pig.Methods The animals were divided into three groups at random and given with Cisplatin(DDP) via i.p.once.The conditions of nausea and vomiting were observed within 8 hours after injection.Results During the experiment,the conditions of nausea and vomiting were not observed in the control animals.0nly 25% of the animals in the group given small dose of DDP((3 mg/kg)) had the behaviors and symptoms of nausea at 382 min after injection but without vomiting.Nausea and vomiting were observed in all the animals in the group given large amount of DDP(4 mg/kg),and the average time of nausea onset observed was 68.25 min,and the mean time of vomiting onset was 138 min.Conclusions Significant dose-dependent effect was observed in the Guizhou miniature pig with homogeneous response,which indicates that Guizhou miniature pig may serve as a new experimental animal for disease model establishment used in the studies of vomiting mechanism,anti-vomiting drug and acupuncture.