松花粉提取物对大鼠实验性高脂血症的预防功能研究

目的 观察松花粉提取物对大鼠实验性高脂血症的预防功能,为松花粉提取物的应用提供实验依据.方法 Wistar大鼠84只,随机分为正常对照组、松花粉提取物0.3、0.15和0.075 g/kg组、辛伐他汀2 mg/kg组、溶媒对照组及高脂模型组.正常对照组,饲喂正常饲料;各给药组在饲喂高脂饲料同时,灌胃（ig）给予相应的药物,溶媒对照组灌胃等容积的葡萄籽油;高脂模型组饲喂高脂饲料同时,灌胃等容积蒸馏水.实验周期为8周,末次给药后12h,检测血清中TC、TG、LDL-C和HDL-C含量.结果 松花粉提取物各组动物血清中TC、TG和LDL-C含量显著低于高脂模型组（P ＜0.05-0.01）;松花粉提取物0.3 g/kg和0.15 g/kg组动脉粥样硬化指数低于高脂模型组（P＜0.05）.结论 松花粉提取物对高脂饲料诱发的大鼠实验性高脂血症有一定的预防功效.

Preventive Effect of Pollen Pini Extract on Experimental Hyperlipoidemia in Rats

Objective To evaluate preventive effect of pollen pini extract on experimental hyperlipoidemia in rats and offer evidence for application of pollen pini extract.Method Eighty four Wistar rats were randomly divided into normal control group,model group,0.3,0.15 and 0.075 g/kg of pellen pini extract groups,simvastatin control group and solvent control group.The rats in normal control group were fed with normal feed.The rats in model group were fed with high fat diet and simultaneously received intragastric administration of distilled water.The rats in solvent control group were fed with high fat diet and simultaneously received intragastric administration grape seed oil.The animals in other groups were fed with high fat diet and simultaneously received intragastric administration the corresponding drug.12 h after the last administration,the content of TC,TG,LDL-C and HDL-C in serum were determined.Result In all doses of pollen pini extract groups,content of TC,TG,and LDL-C in serum were lower than that of model group （P ＜ 0.05-0.01）.In 0.3 g/kg and 0.15 g/kg of pollen pini extract groups,the atherogenic index were lower than that of the model group （P ＜ 0.05）.Conclusion Pollen pini extracts have certain preventive function on experimental hyperlipidemia in rats.