人和动物肿瘤移植于免疫缺陷和正常小鼠脾内后肝转移形成及其机制探讨

为了探讨肝转移形成机制和生物学特性,用人类鼻咽癌(CNE—2Z)和小鼠子宫颈癌(U14)分别在裸鼠和615近交系小鼠脾内移植。结果人类鼻咽癌脾内移植后43—50天有8/10例(80％)在脾一端形成瘤结节的同时肝内也形成转移瘤,其中4例合并肺转移;小鼠子宫颈癌在615小鼠脾内移植后,13—14天处死,结果1和3天切除原发瘤,分别有11％和63％发生了肝转移,而未切除原发瘤有73％发生肝转移。同时有程度不等的肺和淋巴结转移。结果表明凡有足够的癌细胞接种到脾内均可发生原发瘤,继而沿血流系统进入肝脏,在肝内形成转移瘤。

INVESTIGATION OF THE FORMATION OF LIVER METASTASIS AND ITS MECHANISM AFTER INTRAS-PLENIC IMPLANTATION OF HUMAN AND MOUSE TUMOR INTO NORMAL AND IMMUNODIFICIENT MICE

Trying to elucidate the mechamism and biological charateristics of metastatic formation of tumor cell in liver, human nasopharygeal carcinoma (CNE-2Z) and mice uterine cervical carcinoma (U14) were intrasplenicly innoculeted into nude mice and inbred 615 mice respectively. Forty three to fifty days after implantation tumor nodules in spleen and hepatic metastasis were detected in 8/10 cases of CNE-2Z group, of them four cases concurred with pulmonary metastasis. For the U14 group, the mice were sacrificed on the 13rd or 14th day, hepatic metastasis occurred in 73% cases with various degree of pulmonary or/and lymphatic metastasis, of those with primary nodule resection one day or three days after implantation, 11% and 63% demostrated hepatic metastasis respectively. It is suggested that once there is enough tumor cells innoculated into the spleen, a primary tumor would occur in it, and then spreading via blood circulation, secodary metastatic tumor would formed in liver.