不同血清型腺相关病毒在大鼠 DRG神经元转染效果的比较

摘要:目的 比较不同血清型腺相关病毒( AAV5、AAV6、AAV8 和 AAV9) 对背根神经节( DRG) 初级感觉神经元的转导效能。 方法 将 24 只雄性 SD 大鼠随机分为 8 组,分别通过 DRG 直接注射不同血清型腺相关病毒( AAV5、AAV6、AAV8 和 AAV9)载体,各组大鼠分别在转染后第 3 周和第 4 周取材,并在共聚焦显微镜下观测对大鼠 DRG初级感觉神经元的转染效果。 结果 组织切片的荧光结果显示,腺相关病毒各亚型的转染效果如下:转染 3 周,AAV9> AAV6> AAV8 > AAV5;转染 4 周,AAV9> AAV6> AAV8> AAV5。 其中,AAV9 载体对大鼠 DRG 神经元的转导效果较好。 结论 DRG 注射不同血清型腺相关病毒( AAV5、AAV6、AAV8 和 AAV9) 载体,AAV9 对 DRG 神经元的转导效能最佳。

Comparison of the Transfection Effect of Different AAV Serotypes in Rat DRG Neurons

Abstract: Objective To compare adeno-associated virus serotypes ( AAV5, AAV6, AAV8 and AAV9 ) on efficiency of transduction of primary sensory neurons. Method Twenty four male SD rats were randomly divided into 8 groups and the dorsal root ganglion ( DRG) in rats was observed by Olympus-FV1000 after 3 weeks and 4 weeks following direct injection of AAV serotypes ( AAV5, AAV6, AAV8 and AAV9) into the DRG respectively. Result AAV9 was the most effective serotype compared with other AAV serotypes ( AAV5, AAV6 and AAV8) by immunohistochemistry staining. Conclusion Our findings suggest that AAV9 is a highly effective gene delivery vector for primary sensory neurons following direct injection into the DRG