覆盆子对尼古丁戒断焦虑征大鼠的影响

目的 研究覆盆子对尼古丁戒断焦虑的治疗作用，并探讨其作用机制。方法 选取健康 SD大鼠40只，220～250 g，按体质量随机分为5组：空白对照组、模型对照组、药物对照组、覆盆子高剂量组、覆盆子低剂量组。除空白对照组外，其余4组每天皮下注射尼古丁0.4 mg/kg，共7天，制备尼古丁成瘾模型。最后一次注射尼古丁后24 h，药物对照组灌胃地西泮，覆盆子高剂量、低剂量组分别灌胃覆盆子提取物200 mg/kg、100 mg/kg，连续5 d，最后给药1 h后，用高架十字迷宫测其焦虑行为，用HPLC测海马中去甲肾上腺素的含量。结果 在高架十字迷宫中，与空白对照组相比，尼古丁戒断大鼠在开放臂的逗留次数、时间明显减少（P＜0.05）；与模型对照组相比，药物对照组能增加其次数、时间，覆盆子高剂量组作用效果更显著（P＜0.01）；HPLC检测显示：与空白对照组相比，模型对照组大鼠海马中去甲肾上腺素的含量显著升高（P＜0.01），与模型组相比，药物对照组、覆盆子低剂量组、覆盆子高剂量组均可降低海马中去甲肾上腺素含量。结论 覆盆子提取物能减轻尼古丁戒断时大鼠所表现出的焦虑样行为，其抗焦虑症的作用机制可能与降低大鼠脑内海马组织中去甲肾上腺素含量有关。

Effect of Raspberry Extract on Anxiety of Nicotine With d rawal in Rats

Ob jective In the present study， we investigated the effect of raspberry on anxiety-like behavior and the levels of norepinephrine in hippocampus during nicotine withdrawal in rats.Method Sprague-Dawley rats（220—250 g）were treated with 0.4 mg/kg of nicotine or saline by daily subcutaneous injection for 7 days.24 hours after the last injection，rats in the contour group were given diazepam，the experimental group were given raspberry（100 mg· kg-1·d-1、200 mg·kg-1·d-1）， for five days.One hour after the final dose of raspberry， the anxiogenic response was evaluated using an elevated plus maze，the concentrations of norepinephrine in hippocampus were measured by high performance liquid chromatography.Result Rats undergoing nicotine withdrawal exhibited substantial anxiety-like behavior，which was characterized by the decrease in time spent in the open arms of the elevated plus maze，which were greatly attenuated by doses of raspberry.The high performance liquid chromatography analysis revealed that nicotine withdrawal significantly increased norepinephrine levels in the hippocampus.Similar to the result from the elevated plus maze test，the raspberry significantly inhibited the elevation of norepinephrine.Conclusion These result suggest that raspberry attenuates anxiety-like behavior induced by nicotine withdrawal through modulation of the hypothalamic norepinephrine system in the brain.