特异性脱敏法治疗柳树花粉引起的 过敏性鼻炎的实验研究

目的 建立柳树花粉引起的过敏性鼻炎小鼠模型,并对其进行特异性脱敏治疗。 方法 提取柳树花粉蛋白, 对小鼠腹腔注射并滴鼻激发,建立过敏性鼻炎小鼠模型;通过递增变应原剂量对小鼠皮下注射,观察小鼠行为学症 状、鼻黏膜组织形态学变化和检测血清 sIgE 的含量,探索特异性脱敏疗法的效果。 结果 治疗前小鼠出现搔鼻、打 喷嚏、流鼻涕等过敏症状,鼻黏膜中嗜酸性粒细胞明显多于对照组,血清 sIgE 含量高于激发前,成功建立了过敏性 鼻炎小鼠模型;治疗后小鼠过敏症状 逐 渐 减 轻 至 消 失,鼻 黏 膜 中 嗜 酸 性 粒 细 胞 逐 渐 减 少,血 清 sIgE 含 量 减 少。 结论 柳树花粉能够建立过敏性鼻炎小鼠模型,且特异性脱敏法可将其治疗。

Specific Desensitization Therapy Forallergic Rhinitis Caused by Willow Pollen

Objective Establish mouse models of willow pollen allergic rhinitis and immune tolerance treatment of the mouse models. Method Pollen protein was extracted and mouse models of allergic rhinitis was established by intraperitoneal injection of pollen protein and nasal stimulation. By subcutaneous injection of increasing dosage of allergen into mice, the behavioral symptoms of mice were observed, the morphological changes of nasal mucosa were compared, and the content of specific sIgE in serum was detected, so as to study the therapeutic effect of specific desensitization therapy on allergic rhinitis. Result Before treatment, allergic symptoms such as scratching, sneezing and runny nose appeared in mice. Eosinophilsin nasal mucosa were significantly more than those in control group, and the content of specific sIgE in serum was higher than that before stimulation. These results indicate that the allergic rhinitis mouse models induced by willow pollen has been successfully established. After treatment, allergic symptoms of mice gradually decreased to disappear, eosinophils in nasal mucosa gradually decreased, and specific sIgE content in serum decreased. Conclusion Willow pollen can establish allergic rhinitismodelsin mice, and specific desensitization method could treat allergic rhinitis in mice.