Gln 对鸭瘟感染鸭十二指肠免疫调节相关基因表达量的影响

摘要: 目的 为谷氨酰胺( Glutamine，Gln) 在正常鸭及鸭瘟( Duck plague，DP) 模型中对肠道功能及作用途径的研究提供依据。方法 280 只无特定病原体( Specific pathogen free，SPF) 7 日龄雏鸭随机分为 8 组，四组梯度剂量每天灌喂 Gln( 0、0. 5、1、2 g / kg 饲 料) 6d，及另四组灌喂同等梯度剂量 Gln 6 d，于 第 一 天 灌 喂 30 min 后 攻 0. 2 mL2000TCID50 鸭瘟病毒，观察 Gln 对正常雏鸭的免疫促进作用及攻毒鸭免疫应激的缓解作用。测定 Toll 样受体 4( Toll-like receptor 4，TLＲ4) 通路基因表达量。结果 Gln 显著提高正常组 TLＲ4 通路 mＲNA 表达量( P ＜ 0. 05) ，显著降低攻毒组表达量( P ＜ 0. 05) 。结论 Gln 通过 TLＲ4 通路提高正常雏鸭肠道的免疫力，降低鸭瘟病毒( Duck plague virus，DPV) 造成的肠道免疫损伤。

Effect of Glutamine on Expression of Immune-related Genes in Plague Virus Infected Duck Intestine

Abstract: Objective To investigate the effect of Glutamine ( Gln) on the intestine physiological activity and the pathway in normal ducks and the models infected with the plague virus． Method 280 specific pathogen free ( SPF) ducklings were randomly divided into eight groups． Gln was administrated at different doses ( 0，0. 5，1，2 g / kg / feed / d) by oral gavage to the control groups and the model groups infected with 0. 2 mL duck plague virus ( DPV)( 0. 2 mL 2000TCID50) ，respectively． The procedures were performed for 6 days． Effects of Gln in control and model ducklings were detected，and expression of the Toll like receptor 4 ( TLＲ4) pathway genes were determined．Ｒesult Expression of TLＲ4 pathway mＲNAs was increased significantly in normal group ( P ＜ 0. 05) ，but reduced significantly in the model groups ( P ＜ 0. 05) ． Conclusion Gln improves immunity in duckling intestine through the TLＲ4 pathway，and resists the immune-mediated injury caused by DPV．