1 型鸭肝炎病毒强毒株在雏鸭体内分布情况研究

摘要: 目的 利用建立的 Taq 荧光定量 ＲT-PCＲ 检测方法研究鸭肝炎病毒( DHV-1) 强毒株在感染雏鸭体内早期的动态分布规律。方法 本研究采用鸭肝炎病毒强毒株人工感染 3 日龄雏鸭，利用已建立的 Taq 荧光定量 ＲT-PCＲ方法，对接种 24 h 内 DHV-1 强毒株感染雏鸭的组织脏器进行了定量检测。结果 接种后 4 h 即可在雏鸭心、肝、脑、肺、胰腺、回肠、盲肠、直肠、法氏囊和脾脏组织中检测到一定水平的病毒 ＲNA，随着病程发展，ＲNA 拷贝数持续升高，接种 24 h 后，肝脏中的病毒 ＲNA 拷贝数为最高，达到 108. 81 copies/g，心、肺、肾、脾次之，约 107copies /g。结论DHV-1 强毒在雏鸭的早期感染雏鸭的实质器官、免疫器官和消化系统等均具有广泛的嗜性。

Organic Distribution of Duck Challenge Hepatitis Virus Strain in Ducklings

Abstract: Objective To investigate the dynamic distribution of duck challenge hepatitis virus strain ( 161 /79 /V) in experimentally infected ducklings using the Taqman real-time fluorescent quantitative PCＲ． Method Ducklings were infected with the 161 / 79 / V strain at 3 － day age，and the virus ＲNAs were extracted from different organs within 24 hours and quantified using the fluorescent quantitative PCＲ method． Ｒesult DHV ＲNAs were detected in heart，liver，brain，lung，pancreas，ileum，cecum，rectum，bursa and spleen 4 hours after inoculation，and the ＲNA copies continuously increased． The highest copy of the DHV ＲNA was detected in liver ( 108. 81 copies /g) 24 h after inoculation，and the ＲNA copies were somewhat low in heart，lung，kidney and spleen ( about 107 copies /g) ．Conclusion Our study indicates that the challenge DHV strain 161 / 79 / V is tissue or organ ecotropic，such as the solid organ，the immune organs and the digestive system of the infected ducklings．