肥胖,高脂血症性脂肪性肝炎模型的建立

为建立大鼠肥胖、高脂血症性脂肪性肝炎（ＮＡＳＨ）的动物模型。取雄性ＳＤ大鼠１９只，随机人分为模型组（ｎ＝１０）和正常组（ｎ＝９）。模型组喂高脂饮食，即普通饲料基础上加１０％猪油，２％胆固醇。正常组喂普通饲料。观察一般情况血清肝功能、血脂，并行病理学检查。模型组体重、肝指数、血清总胆固醇、游高脂肪酸及转氨酶均显著高于正常组；病理学均出现弥漫性肝细胞脂肪变性，且１００％存在小叶内炎症，８０％出现汇管区

The Establishment of Nonalcohlic Steatohepatitis(NASH) Animal model

Objective To establish a Non\|alcohlic steato\|hepatitis animal model. METHODS Male SD rats were fed with a high\|fat diet for 12 weeks which consisting of the standard diet, 10% lard and 2% cholesterol. After that then the serum lipid, aminotransferase values were detected and with histologically examination. RESULT All model group rats developed NASH which is characterized by significant obesity, elevated serum total cholesterol, free fatty acid and aminotransferase activities with histologically showed hepatocellular macrovesicular steatosis, lobular and portal inflammatory cell infiltration and necrosis. The incidence of NASH in rats with high\|fat diet is 100%. CONCLUSION A new NASH animal model was successfully developed in rats chronically fed with high\|fat diet in 3 months.