小鼠胚胎移植实验方法

目的在小鼠胚胎移植实验中除了胚胎的质量外,希望从受体状况及胚胎移植的技术和方法上摸索出最佳胚胎移植的条件,提高胚胎移植率,为临床胚胎移植提供实验和理论数据。方法从超数排卵或自然发情怀孕3.5 d的母鼠子宫内收集胚胎,在显微镜下观察,以最快的速度将发育与胚龄一致并且形态完好的胚胎捡出,并移植到自然发情2.5 d的小鼠子宫内。结果超数排卵的胚胎质量比自然发情的胚胎质量差,在12个自然发情的受体中有3个怀孕并产下12只仔鼠。结论小鼠胚胎移植与供受体的准备、小鼠的超数排卵、胚胎品质有着密切的关系,胚胎移植的技术和方法对胚胎移植有着至关重要的关系。

Experiment Method of Mouse Embryo Transfer

Objective Besides the embryonic quality, we hope to explore the better technique and method of mouse embryonic transferred, to increase the yield of embryonic transfer. Methods Embryos were collected from both the natural and superovulated estrous mouse of pregnant 3.5 days. Using microscope to collect the embryos that were coincidence in the embryonic development and age, and transferred them to mouse uterus of natural estrous 2.5 days. Result As compared with, superovulation could increase embryonic quantity, however there is no better than natural estrous in embryonic quality. In twelve receptors of natural estrous, three receptors were pregnant and produced twelve mice. Conclusion The factors of affecting embryonic transfer are the donor and receptor, multiple ovulation, embryonic quality score, the number of embryo transferred, the technique and method of embryo transferred.