Smad2基因敲除小鼠冷冻胚胎库的建立

目的 建立Smad2基因敲除小鼠胚胎库。方法 利用OPS法对Smad2基因敲除小鼠胚胎进行玻璃化冷冻保存，并比较不同杂交组合小鼠的超数排卵数、解冻胚胎的复苏率及发育率。结果 两组不同杂交组合（Smad2^＋/－♂×Smad2^＋/－♀和Smad2^＋／－×Smad2^＋／－♀）小鼠平均超排卵数分别为14．13枚和24．60枚；复苏率分别为90．16％和91．67％；囊胚发育率分别为73．08％和77．05％。这些结果表明Smad2基因敲除杂合子母鼠的超排数量明显低于野生型母鼠的超排数量，而二者胚胎解冻后的复苏率和囊胚发育率没有显著差异。因此我们主要通过对野生型小鼠超数排卵，然后与Smad2基因敲除杂合子雄鼠交配的方法获取胚胎，进行玻璃化冷冻保存，现已冻存胚胎1256枚。结论 成功建立了Smad2基因敲除小鼠胚胎库。

Establishment of Embryo Bank for Smad2 Gene Knock-out Mice

Objective To establish embryo bank for Smad2 gene knock-out mice. Methods The OPS vitrification was used to cryopreservation of morula and blastocyst embryos of the Smad2 gene knock-out mice . We compare the results of superovulatory treatment, recovery rate and survival rate in different groups. Results The average numbers of embryo production of Smad2^＋/- and Smad^2＋/＋ were 14.13 and 24.60 respectively after superovulatory treatment. The recovery rates of the two group embryos were 90.16% and 91.67% respectively. The rates of development were 73.08% and 77.05 % in vitro respectively after thawing. The results indicated that the numbers of superovulatory of Smad2^ ＋/- were lower than the ones of Smad2^＋/＋ , the rates of：recovery and the rate of survival were no different between the two group mouse after thawing. We have cryopreserved 1256 embryos of the Smad2 gene knock-out mice by OPS vitrification. Conclusion We establish the embryo bank for the Smad2 gene knock-out mice.