| 不同来源SOD蛋白的稳定性比较 |
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| 引用本文： | 潘剑茹,郑光进,黄文斌,张锦道,刘树滔,饶平凡.不同来源SOD蛋白的稳定性比较[J].福州大学学报(自然科学版),2009,37(5). |
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| 作者姓名： | 潘剑茹 郑光进 黄文斌 张锦道 刘树滔 饶平凡 |
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| 作者单位： | 福州大学生物工程研究所; |
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| 基金项目： | 国家自然科学基金资助项目(30800285); 福建省教育厅科研资助项目(JA08018) |
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| 摘 要： | 纯化制备了来源于猪血的天然SOD,由大肠杆菌体系表达的PTD-SOD1以及由酵母体系表达的重组SOD和PTD-SOD2三种基因重组SOD蛋白,并从pH稳定性、温度稳定性、水浴时间稳定性和外源试剂耐受性几个方面对它们进行了比较.结果表明,重组SOD的稳定性总体上是最强的,PTD-SOD2次之,PTD-SOD1最弱,而天然SOD仅比PTD-SOD1稍强.
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| 关 键 词： | SOD PTD-SOD 稳定性 |
Stability comparison of SOD from different sources |
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| PAN Jian-ru,ZHENG Guang-jin,HUANG Wen-bin,ZHANG Jin-dao,LIU Shu-tao,RAO Ping-fan.Stability comparison of SOD from different sources[J].Journal of Fuzhou University(Natural Science Edition),2009,37(5). |
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| Authors: | PAN Jian-ru ZHENG Guang-jin HUANG Wen-bin ZHANG Jin-dao LIU Shu-tao RAO Ping-fan |
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| Institution: | PAN Jian-ru,ZHENG Guang-jin,HUANG Wen-bin,ZHANG Jin-dao,LIU Shu-tao,RAO Ping-fan(Institute of Biotechnology,Fuzhou University,Fuzhou,Fujian 350108,China) |
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| Abstract: | Wild SOD protein from the blood of pig and three recombinant proteins including recombinant SOD,PTD-SOD1 expressed in Escherichia coli and PTD-SOD2 expressed in yeast were purified respectively.The stability of four SOD proteins were compared with several varieties such as pH,temperature,incubation time and external reagent tolerance.It was proved that recombinant SOD was most stable in many conditions and the stability of PTD-SOD2 was weaker than recombinant SOD but stronger than wild SOD and PTD-SOD1.The ... |
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| Keywords: | SOD PTD-SOD stability |
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