| 梅花鹿骨形态发生蛋白BMP4基因的克隆与序列分析 |
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| 引用本文： | 林峻,李仁宽,林娟,杨捷,叶秀云.梅花鹿骨形态发生蛋白BMP4基因的克隆与序列分析[J].福州大学学报(自然科学版),2012,40(2):275-280. |
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| 作者姓名： | 林峻 李仁宽 林娟 杨捷 叶秀云 |
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| 作者单位： | 福州大学生物科学与工程学院;福州大学酶高效表达国家工程实验室 |
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| 基金项目： | 国家自然科学基金资助项目(20672021);福建省教育厅科研资助项目(JA10023);福州大学人才基金资助项目(XRC1032);福州大学科技发展基金资助项目(2010-XQ-20) |
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| 摘 要： | 通过提取梅花鹿肝脏总RNA以及采用RT-PCR技术,成功克隆梅花鹿骨形态发生蛋白BMP4基因序列,并对其核酸序列、蛋白质序列,功能保守区、亲疏水性进行生物信息学分析,同时构建序列进化树以及部分结构的三维模型.该序列已提交Genbank(索取号:HQ877675).该研究可为日后通过基因工程手段表达重组梅花鹿BMP蛋白并探究其药理作用提供基础.
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| 关 键 词： | 梅花鹿 鹿茸 骨形态发生蛋白 克隆 序列分析 |
Cloning and sequence analysis of a novel bone morphogenetic protein 4 gene from Cervus nippon |
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| LIN Jun,LI Ren-kuan,LIN Juan,YANG Jie,YE Xiu-yun.Cloning and sequence analysis of a novel bone morphogenetic protein 4 gene from Cervus nippon[J].Journal of Fuzhou University(Natural Science Edition),2012,40(2):275-280. |
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| Authors: | LIN Jun LI Ren-kuan LIN Juan YANG Jie YE Xiu-yun |
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| Institution: | 1,2(1.College of Biological Science and Technology,Fuzhou University,Fuzhou,Fujian 350108,China; 2.National Engineering Laboratory for High-efficient Enzyme Expression,Fuzhou University,Fuzhou,Fujian 350108,China) |
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| Abstract: | In this paper,a novel gene encoding BMP4 was cloned successfully from total RNA extracted from the liver of Cervus nippon via RT-PCR.Bioinformatics analyses were performed on the nucleotide sequence,protein sequence,conserved functional region as well as hydrophobicity of the target protein.The phylogenetic tree and simulative 3D structure were also constructed for BMP4.The sequence has been submit to Genbank with the accession number HQ877675.The work reported here provides a basis for expression of recombinant Cervus nippon BMP through genetic engineering as well as exploration of its mode of action in the future. |
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| Keywords: | Cervus nippon antler bone morphogenetic protein cloning sequence analysis |
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