黑暗链霉菌与卡那链霉菌原生质体融合研究

进行了黑暗链霉菌与卡那链霉菌原生质体融合实验 .结果表明 ,将孢子悬浮液接种于种子培养基 ,然后转移到含 0 .5 %Gly的菌丝体培养基收获的菌丝体对形成原生质体最有利 .分别采用 10mg mL、6 0min及 5mg mL、75min的溶菌酶浓度与酶解时间制备黑暗链霉菌与卡那链霉菌原生质体能达到较高的形成率和再生率 .采用单亲灭活卡那链霉菌原生质体的种间融合法 ,得到与亲株形态明显不同的杂合体菌落 ,并筛选到44株妥布霉素产率提高的菌株 ,幅度最高达到 39% .

Study on protoplast fusion between Streptomyces tenebrarius and Streptomyces kanamyceticus

The protoplast fusion between Streptomyces tenebrarius and Streptomyces kanamyceticus was studied. The spores were cultivated in seed media firstly for 24?h, then transferred into mycelia media with 0.5%Gly and cultivated further for 24?h, that high quantity and quality mycelia which were easy to prepare protoplasts can be gotten. The experiment showed that high qulity S.tene protoplasts can be gained in 10?mg/mL lysozyme for 60?min and high qulity S.kana protoplasts can be gained in 5?mg/mL lysozyme for 75?min. By inactivating S.kana protoplasts and then fusing the two kinds protoplasts, there were three kinds recombinants colonies which were quite different in size, color and morphorlogy can be observed in plate.From these different colony, 44 positive recombinants were selected of which the highest tobramycin potency increased 39% than that of the parent strain.