| 苦瓜籽中一种抗胰蛋白肽的分离及抗真菌作用观察 |
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| 引用本文： | 刘璇,时祥柱,叶秀云.苦瓜籽中一种抗胰蛋白肽的分离及抗真菌作用观察[J].福州大学学报(自然科学版),2008,36(1):148-151. |
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| 作者姓名： | 刘璇 时祥柱 叶秀云 |
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| 作者单位： | 福州大学生物工程研究所,福建,福州,350002 |
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| 摘 要： | 利用凝胶过滤层析Sephadex G-75、强阳离子交换色谱SP-650M,从苦瓜籽中分离纯化出一种活性多肽.该多肽在Tricine-SDS-PAGE上显示为单一的谱带,分子量约为6.8 kDa.在经还原剂二硫苏糖醇(DTT)处理后的电泳结果表明该多肽不含有分子间的二硫键.此多肽是一种胰蛋白酶抑制剂,同时具有抗植物致病菌活性,是一种具有双功能作用的多肽.
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| 关 键 词： | 苦瓜籽 纯化 胰蛋白酶抑制剂 抗真菌 |
| 文章编号： | 1000-2243(2008)01-0148-04 |
| 修稿时间： | 2007年3月12日 |
Purification and characterization of a novel antifungal peptide from bitter melon seeds |
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| LIU Xuan,SHI Xiang-zhu,YE Xiu-yun.Purification and characterization of a novel antifungal peptide from bitter melon seeds[J].Journal of Fuzhou University(Natural Science Edition),2008,36(1):148-151. |
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| Authors: | LIU Xuan SHI Xiang-zhu YE Xiu-yun |
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| Institution: | (Institute of Biotechnology,Fuzhou University,Fuzhou,Fujian 35002,China) |
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| Abstract: | A novel antifungal peptide was isolated from bitter melon seeds by extraction with PBS,Sephadex G75 chromatography and strong cation exchange chromatography column(SP-650M).It was identified to be homogeneous by Tricine-SDS-PAGE,and the relative molecular weight was estimated to be about 6.8kDa.It was a trypsin inhibitor and showed antifungal activity against Pythium aphanidermatum,Fusarium oxysporum and Botrytis cinerea.It is expected to be applied to the prevention of insect pests and plant diseases. |
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| Keywords: | bitter melon seeds purification trypsin inhibitor antifungal activity |
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