庆大霉素发酵液中C组分含量测定

本文用荧光薄层扫描法测定庆大霉素发酵液中Ｃ组分含量。用氯仿：甲醇：氨水＝４：５：５下相 为展开剂，在硅胶Ｇ－ＣＭＣ板上分离庆大霉素Ｃ１，Ｃ２及Ｃ１ａ，喷甘油作增荧光试剂，然后用薄层 扫描仪测定其荧光强度。本法具有快速、简便、灵敏度高（Ｃ１，Ｃ２及Ｃ１ａ的检出限量分别为 ０．３、 ０．７及０．２μｇ），线性范围宽及重现性好等优点。也可用于成品及半成品测定。

Determination of Gentamicin C-Component in Fermentation Broth

A fluoro-densitometric method is reported for the quantitative determination of gentamicin C-compount in fermentation broth. The gentamicin C1. C2and C1a were separated on a silica gel CMC plate by using lower phase of chloroform- methanol - concentrated ammonium hydroxide(4:5: 5 ) as developer, and sprayed flu- orescence-cnhancing reagent: glycerol. The fluor-densitometry of C1, C2and C1a were determined with a thin layer chromatographic scanner. This method is quick, simple, high sensitivity (The measurable lowest measures of C1, C2, andC1. are 0. 3, 0. 7 and 0. 2 ug respectively ), and of wide range of linearity and good reproductibility.