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沼水蛙皮肤中抗菌肽的分离纯化与活性测定
引用本文:谢智,黄颖桢,张洋,倪莉.沼水蛙皮肤中抗菌肽的分离纯化与活性测定[J].福州大学学报(自然科学版),2004,32(6):759-762.
作者姓名:谢智  黄颖桢  张洋  倪莉
作者单位:福州大学生物工程研究所,福建,福州,350002
基金项目:福州大学科技发展基金资助项目(QX2000-5XJY-00-01)
摘    要:使用C18反相柱,采用线性梯度洗脱从沼水蛙皮肤分泌物中分离纯化出抗菌肽,应用纸片法测定抗菌活性,并测定抗菌肽氨基酸组成.然后进行序列比对和空间结构预测,从抗菌肽的氨基酸序列和空间结构推测抗菌肽的抗菌机制,并展望了抗菌肽的应用前景.

关 键 词:沼水蛙  皮肤  抗菌肽  测序
文章编号:1000-2243(2004)06-0759-05
修稿时间:2003年12月26

Isolation and bioactivity of antimicrobial peptides from Guenther's frog (Hylarana guentheri) skin
XIE Zhi,HUANG Ying-zhen,ZHANG Yang,NI Li.Isolation and bioactivity of antimicrobial peptides from Guenther''s frog (Hylarana guentheri) skin[J].Journal of Fuzhou University(Natural Science Edition),2004,32(6):759-762.
Authors:XIE Zhi  HUANG Ying-zhen  ZHANG Yang  NI Li
Institution:(Institute of Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, China)
Abstract:Some antimicrobial peptides were isolated from the skin of Guenther's frog Hylarana guentheri, using C18 reversed-phase column and linear gradient elution. After the peptides were detected bioactivity by Filter paper method, analyzed sequence and structure, the mechanism of the antimicrobial peptides were presumed. In addition, the development and application of antimicrobial peptide are also introduced.
Keywords:Guenther's frog  skin  antimicrobial peptide  sequence
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