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Antibodies against analogous heptad repeat peptide HR212 of Newcastle Disease Virus inhibit virus-cell membrane fusion
作者姓名:LI Ying  TIEN Po
作者单位:1. Molecular Virology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China; 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
基金项目:Supported by National Program on Key Basic Research Project (Grant No. 2005CB523001)
摘    要:Membrane fusion is a key step in enveloped virus entry. Highly conserved heptad repeat regions (HR1 and HR2) of Newcastle disease virus (NDV) fusion protein (F) are critical functional domains for viral membrane fusion. They display different conformations in the membrane fusion states and are viewed as candidate targets for neutralizing antibody responses. We previously reported that an analog of heptad repeat peptides HR2-HR1-HR2(HR212) and HR2 could inhibit NDV induced cell-cell membrane fusion. Here, we show that HR212 can induce the production of highly potent antibody in immunized rabbits, which could recognize full length peptides of both HR1 and HR2, and inhibit NDV hemagglutination and NDV entry. These suggest that either HR212 or its antibody could be an inhibitor of virus-induced cell-cell membrane fusion.

关 键 词:NDV    heptad  repeat  domains    immune  response    inhibition  of  viral  entry.

Antibodies against analogous heptad repeat peptide HR212 of Newcastle Disease Virus inhibit virus-cell membrane fusion
Authors:LI Ying  TIEN Po
Institution:1. Molecular Virology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China; 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
Abstract:Membrane fusion is a key step in enveloped virus entry. Highly conserved heptad repeat regions (HR1 and HR2) of Newcastle disease virus (NDV) fusion protein (F) are critical functional domains for viral membrane fusion. They display different conformations in the membrane fusion states and are viewed as candidate targets for neutralizing antibody responses. We previously reported that an analog of heptad repeat peptides HR2-HR1-HR2(HR212) and HR2 could inhibit NDV induced cell-cell membrane fusion. Here, we show that HR212 can induce the production of highly potent antibody in immunized rabbits, which could recognize full length peptides of both HR1 and HR2, and inhibit NDV hemagglutination and NDV entry. These suggest that either HR212 or its antibody could be an inhibitor of virus-induced cell-cell membrane fusion.
Keywords:NDV  heptad repeat domains  immune response  inhibition of viral entry  
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