| 一株高效溶磷小麦内生菌的筛选与鉴定 |
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| 引用本文： | 蒋国彪,马沁沁,方志轩,雍彬,刘欣林,王一丁.一株高效溶磷小麦内生菌的筛选与鉴定[J].四川师范大学学报(自然科学版),2012(1):122-126. |
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| 作者姓名： | 蒋国彪 马沁沁 方志轩 雍彬 刘欣林 王一丁 |
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| 作者单位： | 四川师范大学生命科学学院 |
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| 基金项目： | 国家自然科学基金(30670218)资助项目 |
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| 摘 要： | 从小麦根、茎、叶分离出21株溶磷微生物,筛选出溶磷能力最强的菌株JG-22,经形态特征、生理生化分析及16S rDNA序列分析,确定其为土壤芽孢杆菌(Brevibacillus agri strain).研究表明,JG-22对Ca3(PO4)2溶解能力最强;pH值对菌株JG-22溶磷效果影响最明显,在34～36℃和弱碱性环境下,溶磷效果较好;培养72 h后增加培养时间对有效磷的增量影响不大;Ca3(PO4)2含量在5 g/L以上时加大其含量对有效磷的增量几乎无影响.
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| 关 键 词： | 溶磷 土壤芽胞杆菌 内生菌 筛选 鉴定 |
Screening and Identification of a Phosphate-Dissolving Endophyte from Wheat |
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| JIANG Guo-biao,MA Qin-qin,FANG Zhi-xuan,YONG Bin,LIU Xin-lin,WANG Yi-ding.Screening and Identification of a Phosphate-Dissolving Endophyte from Wheat[J].Journal of Sichuan Normal University(Natural Science),2012(1):122-126. |
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| Authors: | JIANG Guo-biao MA Qin-qin FANG Zhi-xuan YONG Bin LIU Xin-lin WANG Yi-ding |
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| Institution: | (College of Life Science,Sichuan Normal University,Chengdu 610101,Sichuan) |
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| Abstract: | 21 strains of phosphate-dissolving bacteria were separated from wheat roots,stems and leaves and the most powerful phosphate-dissolving was strain JG-22.The characteristics of morphology,physiological and biochemical analysis and 16S rDNA series analysis showed that JG-22 belonged to Brevibacillus agri strain.The study indicated the ability of dissolving Ca3(PO4)2 of JG-22 is the strongest.In 34~36 ℃ and weak alkaline environment,the effect of phosphate-dissolving of JG-22 is evident. |
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| Keywords: | phosphate-dissolving Brevibacillus agri strain endophyte screening identification |
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