pH与铝对蚕豆根尖细胞有丝分裂的交互作用

以蚕豆根尖为材料,运用微核技术研究了不同pH下,Al2(SO4)3溶液对细胞有丝分裂及微核形成的影响.结果表明:二者共同作用会使细胞结构产生明显改变,诱发染色体异常与核固缩,微核率上升.当pH一定时,细胞有丝分裂指数(MI)与微核率(MNF)均随Al浓度增大呈先增后减趋势,MI在Al浓度为10或20 mg/L(仅pH=8.5时)处达最大;MNF在Al浓度10或20 mg/L(仅pH=7.0时)处达到最大.当Al浓度不变时,MI随pH的升高呈先增后减趋势,在pH7.0处达最大;MNF在无铝和Al浓度为10 mg/L组中的最低值出现于pH7.0处,但在高铝组中变化不大.pH与Al均可对细胞产生显著的遗传损伤,二者的交互作用主要表现为遗传损伤的叠加.提高pH可缓解铝毒效应,但不能使之完全消除.

Effect of Interaction of pH and Aluminum on the Mitosis of Root Tip Cells in Vicia faba L

The effect of aluminum sulphate solutions on the mitosis and micronucleus under different pH was studied using V.faba root tip micronucleus assays.The results showed that pH and aluminum induced chromosomal abnormalities and nuclear solid shrinkage,and increased the cell micronucleus.When pH was invariant,the cells mitosis index(MI) and micronucleus frequency(MNF) increased first and then decreased with Al concentration increasing,and MI was the maximum at Al concentration 10 or 20 mg/L(only pH=8.5);MNF was the maximum at Al concentration 10 or 20 mg/L(only pH=7.0).When the Al concentration was constant,MI increased first and then decreased with pH increasing,and the maximum appeared at pH7.0;MNF was minimum at pH7.0 in no Al and Al concentration 10 mg/L group,but MNF was little change in the high Al treatment group.Both pH and Al had a significant genetic toxicity effect which had very complex interactive relationship.The main representation was additive effect genetic damage.Enhencing pH could greatly alleviate aluminum poison effect,but could not eliminate the toxicity of aluminum.