| 乌蕨不同提取液对氧自由基的清除作用 |
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| 引用本文： | 罗娅君,赵正娟,刘思曼,王照丽.乌蕨不同提取液对氧自由基的清除作用[J].四川师范大学学报(自然科学版),2010,33(1). |
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| 作者姓名： | 罗娅君 赵正娟 刘思曼 王照丽 |
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| 作者单位： | 1. 绵阳师范学院,化学与化学工程学院,四川,绵阳,621000
2. 成都市环境保护研究院,四川,成都,610065 |
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| 摘 要： | 采用分光光度法测定了乌蕨3种不同提取液对超氧阴离子自由基(O2-.)和羟自由基(.OH)的清除作用大小.结果表明水提液、质量分数为50%的乙醇提取液和质量分数为80%的丙酮提取液在干样浓度为10.00～50.00μg.mL-1时,对羟自由基的清除率为:水提液(36.85%)乙醇(20.61%)丙酮(18.70%);对超氧自由基的清除率为:丙酮(31.77%)水提液(31.03%)乙醇(24.37%).还利用超声提取法对乌蕨水提液的抗氧化能力进行了研究,结果表明超声水提液对超氧自由基和羟自由基的清除率均大于回流提取液.
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| 关 键 词： | 乌蕨 提取液 氧自由基 清除作用 |
Scavenging Effects of Different Extracts of Stenoloma Chusana (L.) Ching on Oxygen Free Radical |
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| LUO Ya-jun,ZHAO Zheng-juan,LIU Si-man,WANG Zhao-li.Scavenging Effects of Different Extracts of Stenoloma Chusana (L.) Ching on Oxygen Free Radical[J].Journal of Sichuan Normal University(Natural Science),2010,33(1). |
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| Authors: | LUO Ya-jun ZHAO Zheng-juan LIU Si-man WANG Zhao-li |
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| Institution: | LUO Ya-jun1,ZHAO Zheng-juan1,LIU Si-man1,WANG Zhao-li2 (1.College of Chemistry and Chemical Engineering,Mianyang Normal College,Mianyang 621000,Sichuan,2.Chengdu Academy of Environmental Protection,Chengdu 610015,Sichuan) |
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| Abstract: | Scavenging effects of Stenoloma Chusana(L.) Ching on hydroxyl radicals(·OH) and superoxide anion radicals(O_2~-·) of different extracts are studied systemically by spectrophotometry. The water extract, theφ(ethanol)=50% extracts and the φ(acetone)=80% extract have the scavenging effect on hydroxyl radical and superoxide anion radical. Its consistence ranges are 10.00～50.00 μg·mL~(-1). The scavenging effects on ·OH are: water (36.85%)>ethanol (20.61%)> acetone (18.70%), and ones on O_2~-· are: acetone (31.77%)>water (31.03%)>ethanol (24.37%). The antioxygenic activity of water extract is also studied by the ultrasonic wave extraction. The results show that ultrasonic extraction is a highly efficient extraction method compared with the reflux extraction. |
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| Keywords: | Stenoloma Chusana(L ) Ching extracts oxygen free radicals scavenging |
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