| 微生物转化法生产睾酮的发酵工艺改进 |
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| 引用本文： | 郑桂兰,陈小明,王洪钟.微生物转化法生产睾酮的发酵工艺改进[J].江西科学,2007,25(5):565-568,610. |
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| 作者姓名： | 郑桂兰 陈小明 王洪钟 |
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| 作者单位： | 清华大学生物科学与技术系海洋生物技术研究所,北京,100084 |
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| 摘 要： | 原发酵条件中的底物浓度过低,致使发酵液中的产物浓度不高,就提高底物浓度后的其它发酵条件进行了调整,通过正交实验,获得了最佳的发酵培养基配方,最后通过50 L放大发酵获得了成功,发酵液中产物浓度可达3 619 mg/L.
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| 关 键 词： | 微生物转化 睾酮 正交实验 微生物转化法 生产 睾酮 发酵培养基 工艺改进 Conditions 配方 最佳 正交实验 调整 产物浓度 发酵液 底物浓度 发酵条件 |
| 文章编号： | 1001-3679（2007）05-0565-05 |
| 修稿时间： | 2007-07-192007-07-28 |
Improvement for the Frmentation Conditions of Mcrobial Tansformation from Pytosterol to Tstosterone |
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| ZHENG Gui-lan,CHEN Xao-ming,WANG Hong-zhong.Improvement for the Frmentation Conditions of Mcrobial Tansformation from Pytosterol to Tstosterone[J].Jiangxi Science,2007,25(5):565-568,610. |
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| Authors: | ZHENG Gui-lan CHEN Xao-ming WANG Hong-zhong |
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| Institution: | Institute of Mrine Botechnology, Department of Biological Science and Biotechnology, Tsinghua University,Beijing 100084 PRC |
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| Abstract: | The substance concentration in the process we previous reported was too low, and the product concentration in fermentation broth was not high. The substrate concentration was increased in this report, the other fermentation conditions were also adjusted by using orthogonal test, and the product concentration was increased to 3 619 mg/L. A 50 L fermenter was used. |
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| Keywords: | Microbial transformation Testosterone Orthogonal test |
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