| 16S rRNA基因的PCR-DGGE技术分析茶尺蠖幼虫肠道细菌种群结构及多样性 |
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| 引用本文： | 靳亮,王金昌,王洪秀,张贱根,杨罡,靳海燕.16S rRNA基因的PCR-DGGE技术分析茶尺蠖幼虫肠道细菌种群结构及多样性[J].江西科学,2013(6):759-763,829. |
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| 作者姓名： | 靳亮 王金昌 王洪秀 张贱根 杨罡 靳海燕 |
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| 作者单位： | [1]江西省科学院微生物研究所,江西南昌330029 [2]江西省农业科学院农业应用微生物研究所,江西南昌330200 [3]江西省蚕桑茶叶研究所,江西南昌330202 [4]山东省新泰市羊流镇杨家庄小学,山东新泰271200 |
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| 基金项目： | 国家自然科学基金资助项目（31260031）. |
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| 摘 要： | 利用16S rRNA的PCR-DGGE（变性梯度凝胶电泳）技术分析研究了茶尺蠖肠道内细菌的种类与多样性.通过对16S rRNA序列分析,从茶尺蠖野外种群肠适中鉴定了11种细菌,这些细菌分属于沃尔巴克氏菌（Wolbachia）、肠杆菌属（Enterobacter）、沙雷氏菌属（Serratia）、梭菌属（Clostridium）、肠球菌属（Enterococcus）、暂定类群EO1;其中以肠杆菌、沃尔巴克氏菌、沙雷氏菌属最为常见.另外,也鉴定了一种茶树叶绿体上的16SrRNA基因.茶尺蠖幼虫体内的共生菌多样性分析结果,将会为害虫综合治理提供一条全新的探索途径.
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| 关 键 词： | 茶尺蠖 肠道细菌 16S rRNA序列 变性梯度凝胶电泳 多样性 |
Bacterial Community in Midguts of Ectropic oblique Larvae by PCR-DGGE and 16S rRNA Gene Library Analysis |
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| JIN Liang,WANG Jin-chang,WANG Hong-xiu,ZHANG Jian-gen,YANG Gang,JIN Hai-yan.Bacterial Community in Midguts of Ectropic oblique Larvae by PCR-DGGE and 16S rRNA Gene Library Analysis[J].Jiangxi Science,2013(6):759-763,829. |
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| Authors: | JIN Liang WANG Jin-chang WANG Hong-xiu ZHANG Jian-gen YANG Gang JIN Hai-yan |
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| Institution: | 1. Institute of Microbiology,Jiangxi Academy of Sciences,Jiangxi Nanchang 330029 PRC 2. Institute of Agricultural Applied Microbiology, Jiangxi Agricultural Academy of Sciences, Jiangxi Nanchang 330200 PRC; 3. Institute of Sericulture and Tea,Jiangxi Nanchang 330202 PRC; 4. Yangliu Town, Yangjiazhuang Primary School, Shandong Xintai 271200 PRC) |
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| Abstract: | Bacterial community in midguts of Ectropic oblique larvae were studied by PCR-DGGE and 16S rRNA gene library analysis. By 16S rRNA V3 region gene DGGE method,eleven distinct bands were obtained from 16S rDNA amplificons. The bands were purified,sequenced. The sequences were aligned with GenBank database and showed that they were belonged to 6 different genera of bacteria. Phylogenetic analysis showed that the sequences of bacteria belonged to the Wolbachia, Ertterobacter, Serratia, Clostridium,Enterococcus and Tentative genus EO1. The most dominant bacteria group was Enterobacter in the gut. Wolbachia and Serratia followed by it. In addition ,we also found a 16S rRNA gene of Camellia sinensis chloroplast, The analysis of diversity of intestinal microflora from Ectropic oblique larvae will give us more information of microorganism diversity, which provide a new way for the development of bacterial biocontrol agents or implementation as an insecticide. |
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| Keywords: | Ectropic oblique Intestinal bacterium 16S rRNA sequences Denaturing gradient gel electrophoresis （ DGGE） Specie diversity |
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