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大花石榴胚培养再生植株
引用本文:闵炜,陈志萍.大花石榴胚培养再生植株[J].江西科学,2010,28(2):194-195,228.
作者姓名:闵炜  陈志萍
作者单位:上海农林职业技术学院,上海,201600
摘    要:以大花石榴的胚为外植体在MS+BA1+NAA0.2培养基中诱导愈伤并产生不定芽,在MS+BA0.5+NAA0.2培养基中进行芽苗的增殖培养,在1/2MS+NAA0.5+IBA0.5培养基中诱导芽苗生根。试管苗在珍珠岩基质中罩塑料杯保湿炼苗后即可移栽入土中。

关 键 词:组织培养  大花石榴    再生植株

Plantlet Regeneration from the Embryo of Punica granatum cv.Nana in vitro
MIN Wei,CHEN Zhi-ping.Plantlet Regeneration from the Embryo of Punica granatum cv.Nana in vitro[J].Jiangxi Science,2010,28(2):194-195,228.
Authors:MIN Wei  CHEN Zhi-ping
Institution:Shanghai Vocational Technical College of Agriculture &Forestry/a>;Shanghai 201600 PRC
Abstract:The embryos of Punica granatum cv. Nana were used as explants to initiate calluses in the medium of MS+BA1+NAA0.2.The calluses with small buds were cultured in the medium of MS+BA0.5+NAA0.2 for the multiplication of sprouts.The shoots regenerated were rooted in the medium of 1/2MS+ NAA0.5+IBA0.5.The seedlings covered with plastic cups were tamed in the medium of pearlite for a short period and transplanted into soil as the terminal buds had sprouted.
Keywords:Tissue culture  Punica granatum cv  Nana  Embryo  Plantlet regeneration  
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