| 银线草无性系建立的研究 |
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| 引用本文： | 杨贺,梁鑫鑫,翟冠雄,张冬艳,姜长阳.银线草无性系建立的研究[J].江西科学,2011,29(3):324-327. |
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| 作者姓名： | 杨贺 梁鑫鑫 翟冠雄 张冬艳 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁大连,116029 |
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| 基金项目： | 辽宁省高等教育教学改革资助项目(20093040)；辽宁师范大学教学改革资助项目 |
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| 摘 要： | 为满足栽培对种苗的需要,并对野生资源进行保护,本研究以银线草嫩茎为材料,采用组织培养的方法,进行了嫩茎愈伤组织的诱导与分化、试管苗的生根培养,以及移栽和定植的研究,建立起银线草嫩茎的无性系.研究结果表明:MS+ZT 0.2 mg/L+2,4-D 2.0 mg/L是愈伤组织诱导培养和继代培养的理想培养基；MS+BA0.6...
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| 关 键 词： | 银线草 组织培养 无性系 |
The Study on Establishement of the Asexual Line of Chloranthus japonicus |
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| YANG He,LIANG Xin-xin,ZHAI Guan-xiong,ZHANG Dong-yan,JIANG Chang-yang.The Study on Establishement of the Asexual Line of Chloranthus japonicus[J].Jiangxi Science,2011,29(3):324-327. |
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| Authors: | YANG He LIANG Xin-xin ZHAI Guan-xiong ZHANG Dong-yan JIANG Chang-yang |
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| Institution: | (Life Science College of Liaoning Normal University,Liaoning Dalian 116029 PRC) |
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| Abstract: | The aim was to meet the need for cultivation of germchits and protect wild resources.The tender stems of Chloranthus japonicus were used as material to do the research on callus induction and differentiation,rooting and transplanting of tube seedlings,finally establish the clone of Chloranthus japonicus.The results show that MS＋ZT0.2 mg/L＋2,4-D 2.0 mg/L is the optimum medium for callus induction and subculture.The optimum medium for callus and adventitious buds differentiation is MS＋BA0.6 mg/L＋ NAA 0.1 mg/L.1/4MS＋NAA 0.1 mg/L＋IAA0.4 mg/L is the optimum medium for adventitious buds rooting.Colonization of plantlets ismaintained for all biological traits which wild plant has and can flower and bear seeds in the same year.The clone of Chloranthus japonicus was established. |
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| Keywords: | Chloranthus japonicas Tissue culture Clone |
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