高效微生物絮凝剂产生菌的筛选及培养条件优化

从活性污泥中初筛分离出21株产絮凝剂的菌株，复筛得到一株具有较高，较快絮凝活性的微生物产生菌，将其命名为WZU-1。通过培养条件优化，考察了各种因素如碳源、氮源、pH、温度、培养时间、摇床转速对絮凝效果的影响。实验结果表明，WZU-1对高岭土悬浊液的最佳絮凝条件为：碳源为葡萄糖、氮源为尿素、pH为8．0、温度为38℃、摇床转速为200r／min、培养时间为36h，在最佳条件下对4‰高岭土悬浊液的絮凝率达到95．16％。同时对该菌株絮凝性能进行了研究，结果表明该菌絮凝活性物质分布在发酵液离心后的上清液中，为该菌代谢产物；并具有较好的热稳定性。

Screening of Bioflocculant-Producing Strain and Optimizing of Its Culture Conditions

Strains are separated and filtered from activated sludge which is high flocculent bacteria. The series strains are named WZU-1. Then the research was focused on optimization of culture conditions such as carbon source, nitrogen source, pH, temperature, rotational speed and culture time. Amount of WZU-1 were glucose, urea, 8.0,38 ℃, 200 r/min, 36 h. The highest flocculating rate of WZU-1 was up to 95.16% under optimum conditions. Then the flocculanting activities were investigated. The result showed that most of the microbioflocculants were concentrated in the supernatant and the thermal stability of WZU-1 was high.