| S9代谢活化酶系统制备及蛋白含量的测定 |
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| 引用本文： | 何黎黎,杨立开,邓黎,龚涛,孙迅,张志荣.S9代谢活化酶系统制备及蛋白含量的测定[J].西南民族学院学报(自然科学版),2010,36(2):243-245. |
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| 作者姓名： | 何黎黎 杨立开 邓黎 龚涛 孙迅 张志荣 |
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| 作者单位： | 何黎黎,HE Li-li(西南民族大学化学与环境保护工程学院,四川成都,610041);杨立开,邓黎,龚涛,孙迅,张志荣,YANG Li-kai,DENG Li,GONG Tao,SUN Xun,ZHANG Zhi-rong(四川大学华西药学院靶向药物与释药系统教育部重点实验室,四川成都,610041) |
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| 摘 要： | 目的:研究有效的S9代谢活化酶系统的制备方法. 方法:采用苯巴比妥钠作为诱导剂制备大鼠肝S9, 并采用Lowry法测定所制备的S9蛋白含量. 结果:成功制得大鼠肝S9, 并测定其蛋白含量为38.66mg/ml. 结论:苯巴比妥钠作为诱导剂可以成功制备出大鼠肝S9.
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| 关 键 词： | S9制备 蛋白含量测定 |
Preparing metabolic activation system and measuring protein contents |
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| HE Li-li ,YANG Li-kai ,DENG Li ,GONG Tao ,SUN Xun ,ZHANG Zhi-rong.Preparing metabolic activation system and measuring protein contents[J].Journal of Southwest Nationalities College(Natural Science Edition),2010,36(2):243-245. |
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| Authors: | HE Li-li YANG Li-kai DENG Li GONG Tao SUN Xun ZHANG Zhi-rong |
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| Institution: | HE Li-li 1,YANG Li-kai 2,DENG Li 2,GONG Tao 2,SUN Xun 2,ZHANG Zhi-rong 2(1.School of Chemistry , Environment Protection Engineering,Southwest University for Nationalities,Chengdu 610041,P.R.C.,2.Key Laboratory of Drug Targeting , Novel Drug Delivery Systems,West China School of Pharmacy,Sichuan University,P.R.C.) |
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| Abstract: | Objective: To investigate effective methods of producing rat S9(Hepatic post-mitochondrial supernatant).Methods: S9 is prepared with phenobarbital-induced Sprague-Dawley rats,and the protein content is determined by the method of Lowry.Results: Rat liver S9 is successfully prepared,and the protein contents of it are measured to be 38.36mg/ml.Conclusion: Phenobarbital could be used as a induce agent for the producing of rat liver S9. |
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| Keywords: | S9 preparing protein contents measurement |
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