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紫外分光光度法测定藏药HEG胶囊中总黄酮的含量
引用本文:杜朗宇,毛羽,许婧,刘小艺,李艳芬,王萍.紫外分光光度法测定藏药HEG胶囊中总黄酮的含量[J].西南民族学院学报(自然科学版),2009,35(6):1207-1209.
作者姓名:杜朗宇  毛羽  许婧  刘小艺  李艳芬  王萍
作者单位:西南民族大学少数民族药物研究所,四川成都610041
摘    要:目的:建立藏药HEG胶囊中总黄酮的含量测定方法.方法:以芦丁为对照品,于360nm处测定总黄酮的含量结果:总黄酮在5.06—25.30ug·ml^-1范围内,吸收度于浓度呈良好的线性关系(r=0.9999),加样回收率为90.9%.结论:该方法准确,简便,可为藏药HEG胶囊中总黄酮含量的测定提供参考.

关 键 词:芦丁  总黄酮  紫外分光光度法

Determination of total flavonoids contents in Tibetan medicine HEG capsules
DU Lang-yu,MAO Yu,XU Jing,LIU Xiao-yi,LI Yan-fen,WANG Ping.Determination of total flavonoids contents in Tibetan medicine HEG capsules[J].Journal of Southwest Nationalities College(Natural Science Edition),2009,35(6):1207-1209.
Authors:DU Lang-yu  MAO Yu  XU Jing  LIU Xiao-yi  LI Yan-fen  WANG Ping
Institution:(Ethnic Pharmaceutical Institute, Southwest University for Nationalities, Chengdu 610041, P.R.C.)
Abstract:Objective: To establish methods for determination of total flavonoids contents in Tibetan Medicine HEG capsules. Methods: The contents of total flavonoids are measured by ultraviolet spectrophotometry at 360nm with rutin as reference. Result: The total flavonoids show a good linear relationship with the concentration of 5.06-25.30u g.ml^-1 (r=0.9999) and the average recovery is 90.9% (n=6). Conclusion: The present method is accurate, convenient, rapid and suitable for the determination of total flavonoids in HEG capsules.
Keywords:Rutin  total flavonoids  ultraviolet spectrophotometry
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