| 鸭IgG纯化方法比较试验 |
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| 引用本文： | 邱翔,齐雪峰,杨晓燕.鸭IgG纯化方法比较试验[J].西南民族学院学报(自然科学版),2004,30(3):331-333. |
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| 作者姓名： | 邱翔 齐雪峰 杨晓燕 |
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| 作者单位： | [1]西南民族大学生命科学与技术学院，成都610041 [2]四川农业大学动物科技学院，雅安625014 |
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| 摘 要： | 用辛酸-硫酸铵法提取鸭IgG并与硫酸铵法和DEAE纤维素层析法进行比较．结果表明，辛酸-硫酸铵法与DEAE纤维素层析法提取的IgG纯度较高，且辛酸-硫酸铵法简便、快速、节约试剂，所获IgG活性较DEAE纤维素层析法高，适于从大批量样品中提纯IgG。
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| 关 键 词： | 辛酸-硫酸铵法 鸭 IgG |
| 文章编号： | 1003-2843(2004)03-0331-03 |
| 修稿时间： | 2004年3月29日 |
The comparative experiment of purification of duck IgG |
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| Abstract: | A method for purification of duck IgG with caprylic acid and ammonium sulphate
precipitation was developed. And it is compared with ammonium sulphate and DEAE cellulose. The
results suggested that the purification degree of IgG by caprylic acid and ammonium sulphate and
DEAE cellulose is high, and the method of caprylic acid and ammonium sulphate is economical, quick
and simple and could be adapted to purify IgG from a lot of samples. The immunobiolog activity of IgG
is high and stable in the process of the purification. |
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| Keywords: | caprylic acid-ammonium duck IgG |
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