牦牛INHBA基因的克隆及序列分析

根据GenBank检索到的普通牛的INHBA基因序列设计一对特异性引物,以牦牛卵巢组织总RNA为模板,通过RT-PCR技术对牦牛INHBA cDNA进行克隆测序和序列分析.结果表明:扩增片段1360bp,包含1277bp的编码区,编码426个氨基酸.与普通牛相比,牦牛INHBA基因编码区存在2处碱基转化.牦牛与普通牛、绵羊、人、鼠和猪的核苷酸序列同源性分别为99.84%、97.97%、91.41%、87.79%、91.94%,氨基酸序列同源性分别为99.53%、98.82%、95.77%、93.88%、93.88%.蛋白质结构分析显示,INHBA蛋白不易形成α螺旋和跨膜结构,是相对保守的疏水性蛋白.

Molecular cloning and sequence analysis of the INHBA gene in yak （Bos grunniens）

The specific primers were designed according to reference sequence of Bos taurus in GenBank. Using the total RNA extracted fi＇om yak ovarian tissue as template, cDNA sequence of INHBA gene of yak was cloned by RT-PCR and then the sequence was analyzed. The results demonstrated that 1360bp was cloned, with the coding region of 1277bp encoding 426 amino acids. There were two different bases between Bos taurus and Bos grunniens in the coding region. Nucleotide sequcnce similarities of the INHBA gene in Bos grunniens are compared with Bos taurus, Ovis aries, Homo sapiens, Mus musculus, Sus scrota were 99.84%, 97.97%, 91.41%, 87.79%, and 91.94%, respectively, and the amino acid sequence similarities of the INHBA protein in Bos grunniens were 99.53%, 98.82%, 95.77%, 93.88%, and 93.88% respectively. Structural analysis of protein demonstrated that the INHBA protein which was difficult to form Alpha helix and Membrane structure, was relatively conservative hydrophobic proteins.