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鸡白痢沙门氏菌O_(12)特异性噬菌体的分离及生物学特性研究
引用本文:李涵依,车晨,耿亚,等.鸡白痢沙门氏菌O_(12)特异性噬菌体的分离及生物学特性研究[J].西南民族学院学报(自然科学版),2014(2):186-191.
作者姓名:李涵依  车晨  耿亚  
作者单位:西南民族大学生命科学与技术学院,成都610041
基金项目:西南民族大学研究生创新项目
摘    要:采用双层平板培养法从成都某养鸡场的污水样本中分离得到一株鸡白痢沙门氏菌O12(宿主菌)的噬菌体(Ph-1),并对菌斑形态及理化特性进行了初步研究.试验结果显示,纯化后该噬菌体的噬菌斑直径约3mm;经高于60℃温度处理1h后噬菌体失活;PH5-7时该噬菌体可保持较高效价和较强的紫外线耐受性;该噬菌体的最佳感染复数(MOI)为0.01-0.001;一步生长曲线显示该噬菌体的潜伏期为40min,裂解期持续60min.

关 键 词:鸡白痢  噬菌体  沙门氏菌

Study on isolation and biological characterization of bacteriophage of salmonella O12 for pullorum
LI Han-yi,CHE Chen,GENG Ya,LIU Fu-yin,HAO Bao-qing.Study on isolation and biological characterization of bacteriophage of salmonella O12 for pullorum[J].Journal of Southwest Nationalities College(Natural Science Edition),2014(2):186-191.
Authors:LI Han-yi  CHE Chen  GENG Ya  LIU Fu-yin  HAO Bao-qing
Institution:(College of Life Science & Technology, Southwest University tbr Nationalities, Chengdu 610041, P.R.C.)
Abstract:One bacteriophage, named Phi, was isolated from waste-water samples collected from a Chengdu hennery by the double-agar method, and was to be obligate on the salmonella O12 pullorum (Bacillary white diarrhea) that was as host bacteria. The biological catheterization was explored on this bacteriophage. The results indicated that the diameter of Phi phages was about 3ram. When incubated in 60℃ or higher for lh, the phage was inactivated. It was able to keep a high activity at a range of pHS-7. In the UV-test it was demonstrated that Phl possesses a strong ant-UV ability. The best MOx of Phi was 0.01-0.001. It showed that the latent phase and rise phase were 40min and 60 min respectively on the one-step frowth curve.
Keywords:pullorum  bacteriophage  salmonella  
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