| 藏药材红毛五加总皂苷纯化工艺研究 |
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| 引用本文： | 吴春蕾,焦涛,刘圆,孟庆艳.藏药材红毛五加总皂苷纯化工艺研究[J].西南民族学院学报(自然科学版),2009,35(4):785-788. |
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| 作者姓名： | 吴春蕾 焦涛 刘圆 孟庆艳 |
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| 作者单位： | 西南民族大学少数民族药物研究所,四川成都,610041 |
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| 基金项目： | 国家科技支撑计划重点项目，四川省青年基金，国家中医药管理局中医药专项基金 |
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| 摘 要： | 目的：筛选红毛五加总皂苷纯化工艺条件．方法：采用大孔吸附树脂纯化，紫外．可见分光光度法测定红毛五加总皂苷含量．结果：D140型大孔吸附树脂对红毛五加总皂苷的吸附量最大，所以选择D140型大孔吸附树脂分离纯化红毛五加总皂苷．结论：通过大孔吸附树脂富集与纯化，红毛五加总皂苷洗脱率为94．02％．该法可较好地纯化红毛五加总皂苷．
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| 关 键 词： | 红毛五加 总皂苷 大孔吸附树脂 纯化 |
The study on the purification process of total saponins in Acanthopanax giraldii Harms |
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| WU Chun-lei,JIAO Tao,LIU Yuan,MENG Qing-yan.The study on the purification process of total saponins in Acanthopanax giraldii Harms[J].Journal of Southwest Nationalities College(Natural Science Edition),2009,35(4):785-788. |
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| Authors: | WU Chun-lei JIAO Tao LIU Yuan MENG Qing-yan |
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| Institution: | (Ethnic Pharmaceutical Institute of Southwest University for Nationalities, Chengdu 610041, P.R.C.) |
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| Abstract: | Objective： To screen the purification process of total saponins ofA. giraldii Harms. Method： Total saponins of A. giraldii Harms is purified with macroporous adsorption resin and its content is determined by UV-VIS spectropbotometer. Result： The adsorption quantity from total saponins ofA. giraldii Harms by Dl40 macroporous adsorption resin is the most. Conclusion： Enriched and purified by macroporous resin, the elution rate of saponin is 94.02%. This method is simple, accurate, replicate and suitable for the screen of A. giraldii Harms. |
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| Keywords: | A giraldii Harms total saponins macroreticular resin purification |
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