| 用Asmus法测K_m值 |
| |
| 引用本文： | 朱绮琴.用Asmus法测K_m值[J].上海师范大学学报(自然科学版),1988(4). |
| |
| 作者姓名： | 朱绮琴 |
| |
| 作者单位： | 华东师范大学化学系 |
| |
| 摘 要： | 在酶的动力学研究中,K_m能看作为表观电离常数,它在生物化学中是用米氏方程测定,只要酶和底物结合生成一个有色中间络合物,则K_m能用Asmus法测定,本文在酪氨酸酶催化多巴的反应中,用Asmus法计算K_m值,其结果与米氏方程式相一致。
|
| 关 键 词： | 米氏常数(K_m) 酪氨酸酶 3 4-二羟基苯丙氨酸(DOPA) |
The Determination of K_m by Asmus Method |
| |
| ZhU Qiqin.The Determination of K_m by Asmus Method[J].Journal of Shanghai Normal University(Natural Sciences),1988(4). |
| |
| Authors: | ZhU Qiqin |
| |
| Institution: | Department of Chemistry |
| |
| Abstract: | In study of enzyme kinetics, according to the principle of steady state K_m can be regarded as the apparent dissociation constant and determined using Michaelis equation. When enzyme binds with the substrate forming a colored intermediate complex, the value of K_m can be determined by Asmus method. This paper deals with the calculation of K_m in tyrosinase-catalyzed reaction with DOPA by Asmus method. Results obtained agree with that by Michaelis equation. |
| |
| Keywords: | enzyme Michaelis constant(K_m) tyrosinase 3 4-dihydroxyphenylalanine (DOPA) |
| 本文献已被 CNKI 等数据库收录！ |
