| 半连续培养JAL1杂交瘤细胞的生长和代谢 |
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| 引用本文： | 赵渝[],赵冰[],胡昕[].半连续培养JAL1杂交瘤细胞的生长和代谢[J].上海师范大学学报(自然科学版),2003,32(2):64-67. |
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| 作者姓名： | 赵渝[] 赵冰[] 胡昕[] |
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| 作者单位： | 1. 上海师范大学,生命与环境科学学院,上海,200234
2. 上海交通大学,农学院,上海,201101 |
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| 基金项目： | 上海市教委基金(98E05) |
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| 摘 要： | 观察了分泌抗IBDV单抗杂交瘤细胞在改良型5L Celligen细胞反应器中采用半连续培养方式的生长和代谢情况，研究了在该工艺过程中的葡萄糖消耗、单抗的生成、乳酸和氨的产生．同时为培养方案和控制策略进一步的改良确定方向．
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| 关 键 词： | IBDV 杂交瘤 半连续培养 |
| 文章编号： | 1000-5137(2003)02-0064-04 |
| 修稿时间： | 2002年2月25日 |
Growth and Metabolism of JAL1 Hybridoma Cells in Semicontinuous Cultures |
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| ZHAO Yu,ZHAO Bing,HU Xing.Growth and Metabolism of JAL1 Hybridoma Cells in Semicontinuous Cultures[J].Journal of Shanghai Normal University(Natural Sciences),2003,32(2):64-67. |
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| Authors: | ZHAO Yu ZHAO Bing HU Xing |
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| Abstract: | The growth and metabolism of JAL1 hybridoma cells were studied in semicontinuous cultures in improved Celligen bio-reactor (5L).The utilization of glucose and the production of antibody,lactic acid and ammonia were also investigated.Meanwhile,the discussion of operation mode and control strategy in bioreactor culture indicates the direction of their improvement. |
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| Keywords: | IBDV hybridoma cell semicontinuous culture |
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