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经大豆DNA溶液处理后选育的变异水稻基因组RAPD分析
引用本文:李建粤,许燕,等.经大豆DNA溶液处理后选育的变异水稻基因组RAPD分析[J].上海师范大学学报(自然科学版),2001,30(3):46-51.
作者姓名:李建粤  许燕  
作者单位:上海师范大学生命与环境科学学院,上海,200234
基金项目:上海市科技发展基金资助项目(983113008)
摘    要:利用花粉管通道法和浸种及苗期浇灌法,将供体大豆DNA直接导入受体水稻,获得两种水稻变异株系。采用RAPD技术对供体大豆、受体水稻和两种水稻变异株系的基因组进行了多态性分析。所用31种10碱基随机引物中有24种扩增出清晰的DNA条带。分析比较了4个样品DNA扩增条带的相似率。根据试验结果分析,外源大豆DNA导入受体水稻能引起后代基因DNA序列变化,扩大水稻的遗传组成;作者还分析了由外源大豆DNA导入引起受体水稻产生变异现象的原因。

关 键 词:水稻  大豆  外源DNA  随机扩增多态性DNA  RAPD  变异株系  育种  蛋白质
文章编号:1000-5137(2001)03-0046-06
修稿时间:2001年4月27日

RAPD Analysis of t7he Rice Variant Lines Introduced Exogenous DNA From Soybean
LI Jian yue,XU Yan,XU Chao,FAN Shi jing.RAPD Analysis of t7he Rice Variant Lines Introduced Exogenous DNA From Soybean[J].Journal of Shanghai Normal University(Natural Sciences),2001,30(3):46-51.
Authors:LI Jian yue  XU Yan  XU Chao  FAN Shi jing
Abstract:Two groups of rice variant lines increased their protein contents were obtained by introducing exogenous DNA from soybean into rice with soaking seeds, moreover irrigating seedlings, and through the pollen tube pathway after self pollination. Genomes of the donor, the receptor and the two kinds of rice variant lines were analyzed using RAPD assay. 24 polymorphic fingerprints were obtained by using 31 arbitrary primes. Different kinds of amplified bands in RAPD patterns were calculated and analyzed. The results indicated that exogenous soybean DNA introduced into rice could induce variation in the receptor's genome. The cause of variation was analyzed.
Keywords:Rice  Soybean  Exogenous DNA  Random amplified polymorphic DNA (RAPD)
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