| 3种蓑藓属(Macromitrium)植物DNA C-值测定——兼论苔藓DNA C-值研究的生物学意义 |
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| 引用本文： | 解梦,印丽萍,娄玉霞,郭水良.3种蓑藓属(Macromitrium)植物DNA C-值测定——兼论苔藓DNA C-值研究的生物学意义[J].上海师范大学学报(自然科学版),2019,48(2):188-196. |
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| 作者姓名： | 解梦 印丽萍 娄玉霞 郭水良 |
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| 作者单位： | 上海师范大学生命科学学院;上海出入境检验检疫局 |
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| 基金项目： | 国家自然科学基金（31370233，31570208，31860162） |
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| 摘 要： | 分别以小立碗藓(Physcomitrella patens)和水稻(日本晴亚种)(Oryza sativa spp.japonica)为标样,用流式细胞术分别测定了中华蓑藓(Macromirium cavaleriei)、钝叶蓑藓(M.japonicum)和缺齿蓑藓(M.gymnostomum)3种藓类植物的DNA C-值,由此建立了应用流式细胞术测定蓑藓属植物DNA C-值的方法,讨论了影响藓类植物DNA C-值测定结果的因素.最后讨论了苔藓植物DNA C-值变异式样、系统发育和分类学意义,以及今后有关藓类植物DNA C-值研究中值得关注的几个方向.
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| 关 键 词： | 蓑藓 流式细胞术 DNA C-值 藓类植物 |
| 收稿时间： | 2017/4/13 0:00:00 |
Determination on DNA C-values of three Macromitrium species, with comments on biological significances of DNA C-values for bryological research |
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| XIE Meng,YIN Liping,LOU Yuxia and GUO Shuiliang.Determination on DNA C-values of three Macromitrium species, with comments on biological significances of DNA C-values for bryological research[J].Journal of Shanghai Normal University(Natural Sciences),2019,48(2):188-196. |
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| Authors: | XIE Meng YIN Liping LOU Yuxia and GUO Shuiliang |
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| Institution: | College of Life Sciences, Shanghai Normal University, Shanghai 200234, China,Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China,College of Life Sciences, Shanghai Normal University, Shanghai 200234, China and College of Life Sciences, Shanghai Normal University, Shanghai 200234, China |
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| Abstract: | The DNA C-values of three Macromitrium species (including M.cavaleriei,M.japonicum and M.gymnostomum) were determined by flow cytometry by using Physcomitrella patens and Oryza sativa spp. japonica as standard samples,respectively.Thus the method of measuring DNA C-values of Macromitrium species by flow cytometry was established.The factors influencing the measuring result were also discussed.Finnally,variaiton patterns of bryophytes DNA C-values,their phylogenetic and taxonomic significances,and some interesting topics about DNA C-value research were remarked. |
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| Keywords: | Macromitrium flow cytometry DNA C-value moss |
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