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宜兴百合的组织培养和快速繁殖
引用本文:谢杰,余沛涛,王全喜.宜兴百合的组织培养和快速繁殖[J].上海师范大学学报(自然科学版),2006,35(5):76-82.
作者姓名:谢杰  余沛涛  王全喜
作者单位:上海师范大学,生命与环境科学学院,上海,200234
摘    要:以组织培养获得的宜兴百合小鳞茎为试验材料,研究了不同基本培养基、NAA与6-BA的配比、矮壮素(CCC)、2,4-D、活性炭、蔗糖及悬浮培养方法对小鳞茎增殖和膨大的影响.结果表明,最佳诱导培养基为MS培养基,有利于提高分化率的最佳配方为:MS+0.15mg/LNAA+2.0mg/L6-BA,小鳞茎膨大增重的最佳培养基为:MS+0.15mg/LNAA+2.0mg/L6-BA+0.1mg/L CCC+6—8%蔗糖,培养方式为液体振荡培养优于固体培养.

关 键 词:宜兴百合  组织培养  快速繁殖
文章编号:1000-5137(2006)05-0076-07
收稿时间:2006-05-21
修稿时间:2006-05-21

Tissue culture and rapid propagation of Lilium lancifolium Thunb.
XIE Jie,YU Pei-tao,WANG Quan-xi.Tissue culture and rapid propagation of Lilium lancifolium Thunb.[J].Journal of Shanghai Normal University(Natural Sciences),2006,35(5):76-82.
Authors:XIE Jie  YU Pei-tao  WANG Quan-xi
Institution:College of Life and Environment Sciences ,Shanghai Normal University, Shanghai 200234, China
Abstract:The reproduction and extension of bulblets of lily was tested by the way of tissue culture.The effects of the different medium,the combination of NAA and 6-BAm,the chlormequat(CCC),the 2,4-D,the active carbon,the Sucrose and the methods of Suspension culture were studied in the tests.The results showed that MS medium was the most appropriate basic medi- um for inducement of bulblets.The best medium for the scale of lily to reproduction and extension was MS+0.15mg/L NAA+0. 20mg/L 6-BA,the most appropriate medium for adding weight of bulblets of lily was MS+0.15mg/L NAA+2.0mg/L6-BA+1. 0mg/L CCC+6-8% Sucrose.The method of suspension culture was better than that of solide culture.
Keywords:Lilium lancifolium Thunb    tissue culture  rapid propagation
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