| 基于树枝状化合物的聚合酶链反应 |
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| 引用本文： | 王超,沈鹤柏,周丽佳,杨仕平,.基于树枝状化合物的聚合酶链反应[J].上海师范大学学报(自然科学版),2008,37(1):82-88. |
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| 作者姓名： | 王超 沈鹤柏 周丽佳 杨仕平 |
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| 作者单位： | 上海师范大学,生命与环境科学学院,上海,200234 |
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| 基金项目： | 国家自然科学基金
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上海市纳米科技专项基金 |
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| 摘 要： |
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| 关 键 词： | 树枝状化合物 聚合酶链反应 琼脂糖凝胶电泳 PAMAM—DNA聚合体 |
| 文章编号： | 1000-5137(2008)01-0082-07 |
| 修稿时间： | 2007年9月27日 |
Polymerase chain reaction of Polyamidoamine Dendrimers - bound primers |
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| WANG Chao,SHEN He-bo,ZHOU Li-jia,YANG Shi-ping.Polymerase chain reaction of Polyamidoamine Dendrimers - bound primers[J].Journal of Shanghai Normal University(Natural Sciences),2008,37(1):82-88. |
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| Authors: | WANG Chao SHEN He-bo ZHOU Li-jia YANG Shi-ping |
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| Institution: | (College of Life and Environment Sciences,Shanghai Normal University, Shanghai 200234, China) |
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| Abstract: | A polymerase chain reaction （PCR） procedure based on Polyamidoamine Dendrimers （PAMAM） was systemically studied. Such PCR can proceed successfully under optimized condition, with both the two primers bound to the PAMAM surface, respectively. In certain temperature scope, PCR is insensitive to annealing temperature. The nanostructured aggregates prepared via PCR can disperse and congregate reversibly at desired temperatures. The present PCR procedure can extend its use from liquid homogeneous system to nanoparticle surface. |
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| Keywords: | PAMAM PCR agarose gel electrophoresis PAMAM - DNA complexes |
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