大豆总皂甙对运动力竭大鼠心肌抗氧化能力影响的实验研究

目的：研究大豆总皂甙（TS）对运动力竭大鼠心肌抗氧化能力的影响。方法：采用跑台一次力竭急性实验造模。分为四组：安静组,安静加药组,力竭组,力竭加药组。跑台速度30m/min,运动至力竭后立即取材,分光光度法测试心肌组织SOD（超氧化物歧化酶）,CAT（过氧化氢酶）,MDA（丙二醛）,GSH-Px（谷胱甘肽过氧化物酶）,GR（谷胱甘肽还原酶）,GSH（还原型谷胱甘肽）,T-AOC（总抗氧化能力）等指标。结果：与安静组相比,安静加药组SOD、GSH-Px活性显著增强（P〈0.05）,CAT和GR极显著增强（P〈0.01）,力竭组GR极显著升高（P〈0.01）;与力竭组相比,力竭加药组SOD活性、T-AOC显著增强,而MDA显著降低,均具有统计学意义（P〈0.05）,GSH-Px极显著升高（P〈0.01）;与力竭加药组相比,安静加药组GR极显著升高（P〈0.01）。结论：TS能显著的提高运动大鼠心肌组织抗氧化能力,降低MDA生成,延长其力竭运动时间,延缓运动疲劳的发生。

The Experimental Research of Total Soy Saponins on the Effects of Exhausted Rats Myocardium

Objective ：To study the impact on myocardial an tioxidant capacity of soy saponins （TS） in exhaustive exer cised rats. Methods：OneTime Exhaustion acute experimen tal treadmill modeling. Divided into four groups： Control group,TS group, Exhausted group and TS exhausted group. Set Treadmill speed 30m/min, drawn the myocardial tissueimmediately after exhaustive exercise, spectrophotometry testing it＇s cardiac tissue SOD, CAT, MDA, GSHPx, GR, GSH and TAOC. Results： Compared with the Control group ,TS group SOD,GSHPx activity was significantly en hanced（ P 〈0. 05 ）, CAT and GR were significantly en hanced （ P 〈 0. 01 ）, Exhausted group GR significantly in creased （ P 〈0. 01 ） ; compared with Exhausted group, TS exhausted group SOD activity, TAOC significantly en hanced （P〈0.05）, and MDA decreased significantly （ P 〈 0. 05 ）, GSHPx were significantly increased （ P 〈0. 01 ） ; compared with TS exhausted group, TS group GR was significantly increased （ P 〈 0. 01 ）. Conclusions ： TS can significantly improve the antioxidant capacity of myo cardial tissue of exercised rats, decrease its MDA level, ex tend its exercising time, delay the onset of exercise fatigue.