| 趋磁螺菌AMB-l磁小体合成相关基因的克隆及功能分析 |
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| 引用本文： | 谢宝恩,王继雯,慕琦,周伏忠,陈国参.趋磁螺菌AMB-l磁小体合成相关基因的克隆及功能分析[J].河南科学,2009(5). |
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| 作者姓名： | 谢宝恩 王继雯 慕琦 周伏忠 陈国参 |
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| 作者单位： | 河南省微生物工程重点实验室;河南省科学院生物研究所; |
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| 基金项目： | 河南省基础与前沿技术研究计划项目(072300430050) |
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| 摘 要： | 为了更清楚的了解趋磁螺菌产磁小体的合成机理和调节途径,用Tn5转座子诱变的方法筛选得到了2株磁小体合成降低的突变株,并克隆了突变株中被插入失活的基因,分别为编码ABC型Fe3+转移系统中的离子结合蛋白的amb3385基因和功能未知的amb3672基因.互补实验表明携带amb3385和amb3672基因的广宿主载体可以不同程度地恢复突变株中磁小体的合成,证明了D.Schüler关于磁小体合成假说的第一个步骤,即Fe3+从胞外向经由Fe3+转运蛋白运输至了胞内.由于amb3672基因比对时未发现特殊相似基因,其功能尚需进一步研究.
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| 关 键 词： | 磁小体合成 磁小体突变株 趋磁螺菌 Tn5 |
Isolation of Transposon Mutants from Magnetospirillum Magneticum AMB-l and Characterization of Genes Involved in Magnetosome Biosynthesis |
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| Xie Baoen,Wang Jiwen,Mu Qi,Zhou Fuzhong,Chen Guocan.Isolation of Transposon Mutants from Magnetospirillum Magneticum AMB-l and Characterization of Genes Involved in Magnetosome Biosynthesis[J].Henan Science,2009(5). |
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| Authors: | Xie Baoen Wang Jiwen Mu Qi Zhou Fuzhong Chen Guocan |
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| Institution: | 1.Key Laboratory of Microbial Engineering of Henan Province;Zhengzhou 450008;China;2.Institute of Biology;Henan Academy of Science;China |
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| Abstract: | To bring more information about magnetosome synthesis and regulation of Magnetospirillum magneticum,we carried out Tn5 mutagenesis and isolated 2 mutants with decreased magnetosome production.The 2 Tn5-inserted genes from these mutants were cloned and the DNA sequences flanking Tn5 were determined.These genes include amb3385 encoding ABC-type Fe3+ transport system,amb3672 encoding unknown function.Complementation tests showed that amb3385 and amb3672atrA were able to restore the magnetosome production of th... |
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| Keywords: | magnetosome synthesis magnetosome synthesis mutants Magnetospirillum magneticum Tn5 |
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