RP-HPLC法测定新乡种植红豆杉树皮中的三尖杉宁碱

利用反相高效液相色谱法(RP-HPLC)(Agilent TC-C18色谱柱(250.0 mm×4.6 mm,5μm);流动相:乙腈-水(51∶49);流速:1.0 mL/min;检测波长:227 nm,柱温:25℃),测定了新乡种植红豆杉树皮中三尖杉宁碱的含量.结果表明,三尖杉宁碱的线性范围为:0.04~1.4μg,r=0.999;平均回收率为100.40%,RSD=1.07%.首次测定新乡种植红豆杉树皮中三尖杉宁碱平均含量(质量分数)为0.036 0%(n=5).本法操作简便、准确、重现性好,为新乡种植红豆杉提取三尖杉宁碱的测定及其质量控制的提供了方法依据.

Determination of Cephalomannine in Chinese Yew Planted in Xinxiang by RP-HPLC

A method was established for the quantitative determination of bioactive components,adenosine and cordycepin,in culture medium of Cordyceps militaris by reversed-phase high performance liquid chromatography. The separation was performed on Eclipse XDB-C18 column,The mobile phase was 0.02 mol/L phosphate buffer solution-methanol（volume ratio 85∶15）. The flow rate was 1.0 mL/min,and the detection wavelength was 260 nm. The linear measurement range of adenosine was 0.5~100μg/mL（R=0.999 9）;its average recovery was 105.3%;its standard RSD=0.76%（n=5）;and its sample RSD=1.87%（n=5）. The linear measurement range of cordycepin was 0.5~100 μg/mL（R=0.999 9）;its average recovery was 104.0%;its standard RSD=0.95%（n=5）;and its sample RSD=1.23%（n=5）. The method has the characteristics with a wide linear range,a good degree of separation and a good precision. It can be used for quantitative analysis of adenosine and cordycepin in different culture medium for artificial Cordyceps militaris.