| 有机磷降解菌的分离筛选及初步鉴定 |
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| 引用本文： | 王继雯,甄静,谢宝恩,刘莹莹,李冠杰.有机磷降解菌的分离筛选及初步鉴定[J].河南科学,2011,29(1):31-34. |
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| 作者姓名： | 王继雯 甄静 谢宝恩 刘莹莹 李冠杰 |
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| 作者单位： | 1. 河南省微生物工程重点实验室,郑州,450008
2. 河南省科学院,生物研究所有限责任公司,郑州,450008 |
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| 基金项目： | 2009年河南省重大公益科研招标项目 |
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| 摘 要： | 目的:从不同根系土壤分离筛选高效降解有机磷的细菌.方法:通过平板法进行初筛,根据水解圈直径和菌落直径比值大小筛选到水解圈直径和菌落直径比值较大的菌株,进一步通过液体摇瓶培养复筛,对其解磷能力进行了测定,并通过形态特征、生理生化特性和16 S rDNA序列测定等一系列试验时其进行鉴定.结果:分离纯化出10株解磷菌,经平板...
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| 关 键 词： | 有机磷 解磷菌 分离 鉴定 |
Isolation and Screening of Organic Phosphorus-Solubilizing Bacterium and Its Initial Identification |
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| Wang Jiwen,Zhen Jing,Xie Baoen,Liu Yingying,Li Guanjie.Isolation and Screening of Organic Phosphorus-Solubilizing Bacterium and Its Initial Identification[J].Henan Science,2011,29(1):31-34. |
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| Authors: | Wang Jiwen Zhen Jing Xie Baoen Liu Yingying Li Guanjie |
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| Institution: | 1.Key Laboratory of Microbial Engineering of Henan,Zhengzhou 450008,China; 2.Institute of Biology at Henan Academy of Sciences,Zhengzhou 450008,China) |
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| Abstract: | Objective:to isolate and screen organ ophosphate-solubilizing bacterium from the different rhizos-phere and soil.Method:through the plate method for the first screening,we get bacterium with the larger ratio of hydrolysis circle diameter and colony diameter.Further screening in shake culture experiment,it was identified by a series of bacterial colony morphological,physiological characteristics and 16 S rDNA sequences and other tests.Results:of these strains,we get one the better strains XK-1 in organic ophosphate-degradation ability to 22.072 mg/L.It are identified as Bacillus subtilis.Conclusion:Bacillus subtili XK-1 is screened successfully which can excrete high ability of dissolving organic phosphate. |
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| Keywords: | organic phosphorus phosphate-dissolving bacteria isolation identification |
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