| SRAP分子标记分析芫花遗传多样性 |
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| 引用本文： | 王从彦,李晓慧,胡颖,郭二辉,田朝阳.SRAP分子标记分析芫花遗传多样性[J].河南科学,2008,26(5):554-556. |
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| 作者姓名： | 王从彦 李晓慧 胡颖 郭二辉 田朝阳 |
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| 作者单位： | 1. 河南农业大学生命科学学院,郑州,450002
2. 河南省农业科学院园艺所,郑州,450002 |
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| 摘 要： | 采用SRAP分子标记对芫花的遗传多样性进行了分析.结果每对引物组合产生8～20条比较清晰的扩增带.10对引物组合共产生473条扩增带.平均每对引物组合产生47.3条.10对引物组合共产生多态性带92条,每对引物组合产生7～14条,平均为9.2条.每对引物组合产生的多态性带的比例为10.9%～29.1%,平均为19.44%.结果表明,SRAP标记多态性还是较高的,可以用于分析芫花的遗传多样性.
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| 关 键 词： | 芫花 SRAP分子标记 遗传多样性 |
| 文章编号： | 1004-3918(2008)05-0554-03 |
| 修稿时间： | 2008年1月29日 |
Genetic Polymorphism Analysis by Sequence-Related Amplified Polymorphism Markers in Daphne Genkwa |
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| Wang Congyan,Li Xiaohui,Hu Ying,Guo Erhui,Tian Chaoyang.Genetic Polymorphism Analysis by Sequence-Related Amplified Polymorphism Markers in Daphne Genkwa[J].Henan Science,2008,26(5):554-556. |
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| Authors: | Wang Congyan Li Xiaohui Hu Ying Guo Erhui Tian Chaoyang |
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| Institution: | Wang Congyan1,Li Xiaohui2,Hu Ying1,Guo Erhui1,Tian Chaoyang1(1.College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China,2.Institute of Horticulture,Henan Academy of Agriculture Sciences,China) |
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| Abstract: | Polymorphism of Daphne genkwa were analyzed with SRAP markers.There were 8～20 clear bands per primer pairs.About 473 amplified bands and 92 polymorphic bands were detected by 10 SRAP primer pairs.There were 7～14 clear polymorphic bands per primer pairs.The number of amplified bands and polymorphic bands per primer pairs were 47.3 and 9.2 respectively.The percentage of polymorphic bands was 10.9 %~29.1 %,average 19.44 %.All these results showed that the Genetic diversity of Daphne genkwa is low and the polymorphism of SRAP markers is highely,and could be widely used in analyzed plants such as Daphne genkwa. |
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| Keywords: | Daphne genkwa SRAP molecular marking inherit diversity |
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