| 利用原生质体融合技术筛选红霉素优良菌株 |
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| 引用本文： | 张琪,杨华,朱德育,李武德,周桂香.利用原生质体融合技术筛选红霉素优良菌株[J].河南科学,2009,27(7):809-812. |
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| 作者姓名： | 张琪 杨华 朱德育 李武德 周桂香 |
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| 作者单位： | 河南天方药业股份有限公司,河南,驻马店,463000 |
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| 摘 要： | 以红霉素高产菌HE-08-6及低产优组分菌HA-08-20为出发菌株,其原生质体经紫外诱变后在适宜条件下进行融合,再生培养后挑取融合子89株,经筛选得到3株具有二亲本优良性状的高产菌株HEA-08-01,HEA-08-02,HEA-08-03.其化学效价较出发菌株HA-08-20提高约25%,产品的红霉素A组分含量较出发菌株HE-08-6提高约26%.传代培养证明该菌株具有一定的遗传稳定性,在50L-Fus智能发酵罐上连续3批发酵结果表明,该优良菌种在提高红霉素产品质量及效价上取得较大进展,具有推广应用价值.
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| 关 键 词： | 红霉素 原生质体融合 紫外诱变 |
Screening Fine Strains of Erythromycin by Protoplast Fusion Technique |
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| Zhang Qi,Yang Hua,Zhu Deyu,Li Wude,Zhou Guixiang.Screening Fine Strains of Erythromycin by Protoplast Fusion Technique[J].Henan Science,2009,27(7):809-812. |
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| Authors: | Zhang Qi Yang Hua Zhu Deyu Li Wude Zhou Guixiang |
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| Institution: | Henan Topfond Pharmaceutical Co. Ltd;Zhumadian 463000;Henan China |
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| Abstract: | Three mutant strain HEA-08-01 HEA-08-02 and HEA-08-03 with fine character was selected from fused parent strains HE-08-6 and HA-08-20 after treatment of their protoplasts using UV light . The production biological potency increased about 25 percent and the content of component ERY A content increased about 26 percent compared with their paraents. This three mutant strain had genetic stability after cultured for several generations,and had high practicability after applied in 50 L-Fus fermentor for three bat... |
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| Keywords: | erythromycin protoplast fusion UV mutagenesis |
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