| 垂柳的组织培养及植株再生体系研究 |
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| 引用本文： | 袁秀云,郭利勇,马杰,张仙云.垂柳的组织培养及植株再生体系研究[J].河南科学,2008,26(1):42-44. |
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| 作者姓名： | 袁秀云 郭利勇 马杰 张仙云 |
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| 作者单位： | 1. 郑州师范高等专科学校,生物技术研究所,郑州,450044
2. 河南省经济林和林木种苗工作站,郑州,450008 |
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| 基金项目： | 河南省郑州市科技攻关项目 |
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| 摘 要： | 对垂柳愈伤组织和植株再生体系进行了研究,结果显示茎段比较适合诱导愈伤组织,质量浓度为1.0mg/L和2.0mg/L的6-BA均能诱导垂柳茎段愈伤组织;MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+KT 0.5 mg/L的激素配比最为适合胚状体和芽的分化;MS+6-BA 1.0 mg/L+Ac 0.3%培养基能促使垂柳根的形成,且能促使芽生长,最终形成幼苗。
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| 关 键 词： | 垂柳 组织培养 植株再生 |
| 文章编号： | 1004-3918（2008）01-0042-03 |
| 收稿时间： | 2007-10-11 |
| 修稿时间： | 2007年10月11 |
Research on Tissue Culture and Regeneration of Salix babylonica |
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| YUAN Xiu-yun,GUO Li-yong,MA Jie,ZHANG Xian-yun.Research on Tissue Culture and Regeneration of Salix babylonica[J].Henan Science,2008,26(1):42-44. |
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| Authors: | YUAN Xiu-yun GUO Li-yong MA Jie ZHANG Xian-yun |
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| Institution: | 1.Institute of Biology Technology;Zhengzhou Teacher's College;Zhengzhou 450044;China;2.Economic Forest and Tree Seeding Center of Henan;Zhengzhou 450008;China |
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| Abstract: | In this paper the tissue culture and plant regeneration of Salix babylonica was researched.The results show that the stem is optimum to induce callus;The 6-BA concentration of 1.0 mg/L and 2.0 mg/L can improve callus of Salix babylonica;MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+KT 0.5 mg/L is the optimum medium for embryoid and bud induction;MS+6-BA 1.0 mg/L+Ac 0.3 % can not only grow root,but also improve bud,and in final regenerated plant. |
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| Keywords: | Salix babylonica tissue culture plant regeneration |
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