| HPLC法测定荧光增白剂CBS-127的含量 |
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| 引用本文： | 李自红,魏悦,范毅,董建军.HPLC法测定荧光增白剂CBS-127的含量[J].河南科学,2013(1). |
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| 作者姓名： | 李自红 魏悦 范毅 董建军 |
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| 作者单位： | 河南省科学院生物技术开发中心,郑州 450002 |
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| 摘 要： | 建立了高效液相色谱法测定荧光增白剂CBS-127含量的方法.采用Sunfire C18(4.6 mm×150 mm,5μm)色谱柱,流动相为甲醇-水(90%∶10%),流速为1.0 mL/min,紫外检测波长为356 nm,柱温40℃.CBS-127在0.5～2.5μg范围内有良好线性关系(r=0.999 9);加样回收率为99.78%,RSD值为0.7%(n=6).该方法精密度高、重现性好、结果准确可靠,为荧光增白剂CBS-127的质量评价提供了有效的方法.
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| 关 键 词： | 高效液相色谱 荧光增白剂 CBS-127 |
Determination of the Component of Fluorescent Whitening Agent CBS-127 by HPLC |
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| Abstract: | The method for determinating the content of Fluorescent Whitening Agent CBS-127 by HPLC was established.Sunfire C18 column(4.6 mm×150 mm,5 m) was used with methanol-water(90% ∶10%) as the mobile phase.The flow rate of the mobile phase was 1.0 mL/min.The detection wavelength was 356 nm.The column temperature was 40 ℃.There was good linear relationship in the range of 0.5～2.5 μg(r = 0.999 9).The recovery rate was 99.78% and RSD value is 0.7%(n=6).This method is high accuracy,good reproducibility,reliable results,which is an effective method for the Fluorescent Whitening Agent CBS-127. |
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| Keywords: | HPLC Fluorescent Whitening Agent CBS-127 |
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