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| 柱状田头菇原生质体制备条件的实验研究 | |
| 引用本文： | 周伏忠,陈晓飞,孙玉飞,宁萌,陈国参.柱状田头菇原生质体制备条件的实验研究[J].河南科学,2012,30(4):440-443. |
| 作者姓名： | 周伏忠 陈晓飞 孙玉飞 宁萌 陈国参 |
| 作者单位： | 1. 河南省微生物工程重点实验室,郑州450008;河南省科学院生物研究所有限责任公司,郑州450008 2. 河南省科学院生物研究所有限责任公司,郑州,450008 3. 河南省微生物工程重点实验室,郑州,450008 |
| 基金项目： | 河南省基础和前沿技术研究项目（112300413210）资助; 高新技术产业化项目（112105000030）资助 |
| 摘 要： | 对影响柱状田头菇菌株原生质体制备的条件进行了研究,这些条件包括不同的培养基、不同的温度、不同的pH、不同的酶解时间、不同的菌龄以及不同的渗透压稳定剂.结果表明:液体摇瓶振荡培养5 d的菌丝,以0.6 mol/L的甘露醇或MgSO4作为渗透压稳定剂,采用2%的真菌溶壁酶,在pH为6、温度为31℃的条件下酶解2 h,柱状田头菇菌株的原生质体制备率最高,可达到2.31×106个/mL. |
| 关 键 词： | 柱状田头菇 原生质体 制备 |
Studies on Preparation of Protoplast of Agrocybe cylindracea Strain |
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| Zhou Fuzhong , Chen Xiaofei , Sun Yufei , Ning Meng , Chen Guocan.Studies on Preparation of Protoplast of Agrocybe cylindracea Strain[J].Henan Science,2012,30(4):440-443. | |
| Authors: | Zhou Fuzhong Chen Xiaofei Sun Yufei Ning Meng Chen Guocan |
| Institution: | 1.Henan Key Laboratory of Microbial Engineering,Zhengzhou 450008,China; 2.Biology Institute Co.,Ltd.Henan Academy of Science,Zhengzhou 450008,China) |
| Abstract: | In this paper,the protoplast preparation conditions of Agrocybe cylindracea strain were studied,these conditions include different media,different temperatures,different pH,different reaction time,different mycelial age and different osmotic stabilizers.The results showed that：after the mycelium cultured for 5 days in liquid oscillation shaking,with 0.6 mol/L mannitol or MgSO4 as osmotic stabilizer,and a concentration of 2% of the fungal wall dissolving enzyme（lywallzyme） in the pH6.0,temperature 31 ℃ for 2 hours reaction treatment,the yield of protoplast of Agrocybe cylindracea strain was highest and can reach 2.31×106 /mL. |
| Keywords: | Agrocybe cylindracea protoplasts preparation |
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