| 产蛋白酶和淀粉酶菌株的筛选及其发酵产酶特性研究 |
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| 引用本文： | 陈国参,宁萌,刘德海,马焕,权淑静.产蛋白酶和淀粉酶菌株的筛选及其发酵产酶特性研究[J].河南科学,2013(12):2149-2152. |
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| 作者姓名： | 陈国参 宁萌 刘德海 马焕 权淑静 |
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| 作者单位： | 河南省科学院生物研究所有限责任公司,郑州450008 |
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| 基金项目： | 河南省重大公益科研项目（111100910800） |
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| 摘 要： | 利用酪蛋白固体平板分离培养基、淀粉平板分离固体培养基初筛，液体发酵产酶培养基摇瓶复筛相结合，从实验室保藏的真菌茵株中筛选出一株产蛋白酶的真茵菌株和一株产淀粉酶的真茵茵株，对其固态发酵特性进行了研究．结果表明：蛋白酶产酶高峰为24h，酶活力达到3760tJ／g；淀粉酶产酶高峰为32h，酶活力达到0．405u／g．
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| 关 键 词： | 蛋白酶 淀粉酶 茵株筛选 |
Screening of Protease and s-amylase Producing Strains and Its Fermentation Properties |
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| Chen Guocan,Ning Meng,Liu Dehai,Ma Huan,Quan Shujing.Screening of Protease and s-amylase Producing Strains and Its Fermentation Properties[J].Henan Science,2013(12):2149-2152. |
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| Authors: | Chen Guocan Ning Meng Liu Dehai Ma Huan Quan Shujing |
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| Institution: | 1. Institute of Biology Co. Ltd., Henan Academy of Science, Zhengzhou 450008, China： 2. Henan Engineering Research Center of Industrial Enzymes, Zhengzhou 450008, China) |
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| Abstract: | The strains Aspergillus niger with higher protease production,Aspergillus oryzae with higher a-amylase production were screened from fungal strains in laboratory,through the preliminary screening with Casein plate and Soluble starch plate solid culture medium, the second screening with liquid fermentation enzyme production medium of shake flash fermentation. It was studied on producing enzymes by solid-state fermention. The results showed that the protease activity was 3760 U/g for fermentating 24 h, while the fungal c~-amylase activity was 0.405 U/g for fermentating 32 h. |
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| Keywords: | protease： fungal a-amylase strain screening |
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